【正文】 fluid Laser capturemicrodissected tissue Saliva Breast interstitial fluid Liver cyst fluids Tear Cell lysates Tissue lysates Other 抗體 芯片 蛋白 芯片 抗體 重組 蛋白 ELISA/EIA 定制產(chǎn)品 多肽 根據(jù)客戶需求 靈活多變 產(chǎn)品定制 多種多樣 ?RayBiotech公司抗體抗原庫的資源 ?客戶擁有的原材料 ?市場上可購買到 定制服務(wù) RayBiotech Custom Array Raybiotech 亞特蘭大總部 廣州瑞博奧 謝謝！ 聯(lián) 絡(luò) 我 們 廣州瑞博奧生物科技有限公司 電 話 : 020 3205 1923, 3229 0761 （免費 : 4000085918） 傳 真 : 020 3205 3719 銷 售 部 : 網(wǎng) 址： 地 址 : 廣州市科學城掬泉路 3號廣州國際企業(yè)孵化器 F區(qū)四樓 Questions amp。 Answer Quantibody174。 vs. Luminex xMAP 1) No dedicated equipment needed 2) More Flexibility 3) Higher density arrays 4) Larger choice of targets 5) Better reproducibility 6) Better suited for screening and validation of biomarkers Antibody Arrays vs. Bead Assays （ luminex） ? Fewer unwanted Ab–Ab interactions – Beadconjugated antibodies can interact freely in solution – Capture antibodies affixed to array solid support – Easier to integrate more antibodies per assay ? More flexibility and more options – Multiple detection formats: chemiluminescent,fluorescent or chromogenic – No dedicated equipment to purchase – Can screen for hundreds of proteins, or focus on a few – Can switch to quantitative multiplex after preliminary screening for relative expression Stem Cell Array Coming soon, not finished yet 0867 Differential Expression of Cytokines in Oral Wash of HIVinfected Patients J. CHANDRA1, R. JUREVIC2, P. MUKHERJEE1, M. LEDERMAN3, and M. GHANNOUM1, 1Center for Medical Mycology, Department of Dermatology, Case Western Reserve University, Cleveland, OH, 2Department of Biological Sciences, School of Dental Medicine, Case Western Reserve University, Cleveland, OH, 3School of Medicine, Case Western Reserve University, Cleveland, OH Objectives: To determine oral cytokine profile (OCP) of healthy subjects and HIVinfected patients, and identify cytokines present differentially in HIVinfected patients. Methods: OCP was characterized using oral wash samples (OWS) obtained from 12 healthy subjects and 12 HIVinfected patients, collected using IRBapproved protocol. Initially, a qualitative screening of cytokines was performed using cytokine antibody array membranes (RayBiotech) in the absence or presence of endogenous protease inhibitors (PIs). Next, the levels of specific cytokines in OWS were quantified using 16plex ELISA. Association of changes in cytokine levels with CD4 counts and viral loads in HIVinfected patients was also determined. Results: Nine cytokines were detected in OWS obtained from healthy or HIVinfected subjects, and were not degraded by salivary proteinases. The detected cytokines included: ENA78 (epithelial neutrophilactivating peptide), GRO, IL8 (Interleukin8), EGF (Epidermal growth factor), MCP1 (Monocyte chemotactic protein1), TIMP1 and 2 (Tissue inhibitor of metalloproteinases), IL1a, and angiogenin. Among these cytokines, levels of GRO and IL8 were elevated in 25% (3/12) HIVinfected patients, while MCP1 and ENA78 were low or absent in 33% (4/12) and 50% (6/12) of these patients, respectively, pared to healthy individuals. ELISA studies revealed that IL5 level was significantly higher in HIVinfected patients pared to healthy subjects ( 177。 pg/mL and 177。 pg/mL, P = ). Although a trend showing increased levels of IL1b, IL13, and TNFb was observed in HIVinfected patients, these were not statistically significant (P = .063, .054 and .06, respectively). Among HIVinfected patients, levels of TNFb and IL5 tended to increase with increase in CD4 counts, while that of TNFb decreased with increase in viral loads. Conclusion: Our study identified the cytokine profile of OWS in healthy and HIVinfected subjects, and those that are differentially expressed in the oral cavity of HIVinfected patients. Funding Support: NIH/NIDCR AIU0168636 A: normal control B: CHB C: HCC