細胞工程論文摘要及總結(jié)(編輯修改稿)
2025-07-21 00:17 本頁面
【文章內(nèi)容簡介】 牛血清)+ mmol/L 2流基乙醉等為基礎(chǔ)培養(yǎng)液，添加LIF. SCF, bFGF為培養(yǎng)液，分離培養(yǎng)的部分羊水細胞具有ES細胞特性，細胞克隆形成集落，細胞結(jié)構(gòu)致密，界限不清，部分細胞AP, OCT4, , OCT4, SSEA1等均為胚胎性多能性干細胞的特異性標志，羊水細胞可能是胚胎性多能性干細胞的又一個潛在來源和途徑，其可能來源于脫離正常遷移定居路途的原始生殖細胞(PGCs )或為間質(zhì)干細胞或上皮千細胞的祖細胞。羊水源胚胎性多能性千細胞的發(fā)現(xiàn)可以避免人類ES細胞研究的論理學問題，可為胎兒和人類重大疾病的治療提供大l}的種子細胞。關(guān)鍵詞:人。羊水。胚胎(ES)干細胞:OCT4。多能性Culture pH and osmolality influence proliferation and embryoid body yields of murine embryonic stem cells Muhammad A. Chaudhrya, b, Bruce D. Bowenb and James M. Pireta, baMichael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z4, Canada bDepartment of Chemical and Biological Engineering, University of British Columbia, 2360 East Mall, Vancouver, BC V6T 1Z3, Canada Received 16 October 2008。 revised 27 February 2009。 accepted 6 March 2009. Available online 19 March 2009. AbstractThe realization of many stem cell based regenerative therapies will depend on culture production. Murine embryonic stem cells (mESC) provide a practical model for stem cell process research as they can be readily obtained at relatively high numbers and purities. However, an understanding of their environmental tolerance ranges is still lacking. These tolerance ranges were explored using an embryoid body (EB) formation assay as a functional measure of mESC maintenance. The mESC were exposed to wide ranges of initial medium glucose, glutamine, ammonium, lactate, pH and osmolality conditions. Within the mon ranges of conventional maintenance cultures, the only environmental variables that significantly influenced EB yields were pH and osmolality. Dose–response experiments with these two variables revealed that, within 48h, the EB yield was, for example, 3fold decreased (p) when mESC were cultured either at an initial pH of or an osmolality of 400mOsm/kg pared to a medium at pH and 300mOsm/kg. This decline was due to decreases in both the growth rate and in the fraction of EBforming cells More extreme pH ( or ) as well as osmolality (200 or 500mOsm/kg) conditions reduced the EB formation potential to even lower levels. The decreased growth rates and EB forming potential of mESC cultured at pH or for 24 or 48h, when returned to pH medium, r
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