檢測(cè)器的種類(lèi)及選擇方法(編輯修改稿)
2025-06-14 21:14 本頁(yè)面
【文章內(nèi)容簡(jiǎn)介】 器 (ECD) 電化學(xué)檢測(cè)器是測(cè)量物質(zhì)的電信號(hào)變化，對(duì)具有氧化還原性質(zhì)的化合物，如含硝基、氨基等有機(jī)化合物及無(wú)機(jī)陰、陽(yáng)離子等物質(zhì)可采用電化學(xué)檢測(cè)器。包括極譜、庫(kù)侖、安培和電導(dǎo)檢測(cè)器等。前三種統(tǒng)稱(chēng)為伏安檢測(cè)器，用于具有氧化還原性質(zhì)的化合物的檢測(cè)，電導(dǎo)檢測(cè)器主要用于離子檢測(cè)。其中安培檢測(cè)器 (AD)應(yīng)用較廣泛，更以脈沖式安培檢測(cè)器最為常用。 原理：在兩電極之間施加一恒定電位，當(dāng)電活性組分經(jīng)過(guò)電極表面時(shí)發(fā)生氧化還原反應(yīng) (電極反應(yīng) )，電量 (Q)的大小符合法拉第定律 Q＝ nFN。因此，反應(yīng)的電流 (I)為： I＝ nFdN／dt，式中 n為每摩爾物質(zhì)在氧化還原過(guò)程中轉(zhuǎn)移的電子數(shù)， F為法拉第常數(shù)， N為物質(zhì)的摩爾數(shù)， t為時(shí)間。當(dāng)流動(dòng)相的流速一定時(shí)， dN／ dt與組分在流動(dòng)相中的濃度有關(guān)。 company name 電化學(xué)檢測(cè)器 (ECD) 優(yōu)點(diǎn)：靈敏度很高，尤其適用于痕量組分分析。 缺點(diǎn)：干擾比較多，如生物樣品或流動(dòng)相中的雜質(zhì)、流動(dòng)相中溶解的氧氣及溫度的變化等都會(huì)對(duì)其產(chǎn)生較大的影響。電極壽命有限，對(duì)溫度和流速的變化比較敏感。 適用范圍：應(yīng)用范圍廣，凡具氧化還原活性的物質(zhì)都能進(jìn)行檢測(cè)，本身沒(méi)有氧化還原活性的物質(zhì)經(jīng)過(guò)衍生化后也能進(jìn)行檢測(cè)。 company name (3) Ju Qiu, Nozomi Saito, Mai Noguchi, Keiichi Fukui, Kayo Yoshiyama, Kazusato Matsugano,Norihiko Terehara, and Toshiro Matsui..Absorption of 6OCaffeoylsophorose and Its Metabolites in Sprague Dawley Rats Detected by Electrochemical DetectorHighPerformance Liquid Chromatography and Electrospray Ionization TimeofFlightMass Spectrometry Methods[J]. Agric. Food Chem. 2021, 59, 6299–6304 (3)Absorption andmetabolism of a natural pound, 6O caffeoylsophorose (CS) from acylated anthocyanins in a red vinegar fermented with purple sweetpotato, were clarified. The absorption of CS and conjugated CS in blood from orally administrated SpragueDawley rats at a dose of 400 mg/kg was investigated by electrochemical detectionhigh performance liquid chromatography. As a result, CS was successfully detected in rat plasma (AUC06h, ( nmol h/mL) and was found to be an intact absorbable polyphenol. In addition, half of the absorbed CS was detected as its conjugates (AUC06h, ( nmol h/mL) as well as caffeic and ferulic acids from CS. 電化學(xué)檢測(cè)器 (ECD) company name 電化學(xué)檢測(cè)器 (ECD) Figure 6. Typical ECD HPLC chromatograms of standard (A) and rat plasma before (B) and 30 min after (C) a single oral administration of 400 mg/kg CS to SD rat. Peaks 1, 2, and 3 denote CS, caffeic acid, and ferulic acid, respectively. HPLC and ECD conditions are described in the Materials and Methods. company name Microfabricated Electrochemical Detector for HighPerformance Liquid Chromatography (4) (4)Evan T. Ogburn, Michael Dziewatkoski, Don Moles, Jay M. Johnson, and William R. Heineman. Microfabricated Electrochemical Detector for HighPerformance Liquid Chromatography[J]. Anal. Chem. 2021, 83, 6963–6970 A microfabricated electrochemical cell has been developed as a disposable detector for flow injection analysis (FIA) and highperformance liquid chromatography (HPLC).The simplicity of the fabrication process allows this detector to be used as a lowcost, disposable device that can be replaced easily if its performance degrades rather than disassembling the detector and polishing the electrode surface, which is the usual procedure. The detector
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