科大高分子課件四聚合物在生物高分子分離中的應用(完整版)
【正文】 水或疏水之分。 ? 胺類：三乙醇胺、三乙胺、 N乙基二乙醇胺， N, N, N’, N’四甲基 1, 3 丁二胺（ TMBD）等。 Madabhushi R S. Electrophoresis， 1998， 19： 224～ 230. Verzola B, Gelfi C, Righetti P G. J Chromatogr A， 2022，874： 293～ 303. 羥乙基纖維素的改性 S iOS iOS iOS iOS iOHOHOHO OO H O HN ( C H 3 ) 3 N ( C H 3 ) 3H y d r o g e n b o n dI o n i c b o n dC a t i o n i z e d h y d r o x y e t h y l c e l l u l o s eON a O HN + ( C H 3 ) 3 C l OOOO HH OO O HO H O O O HOO HO OOOO HH OOO H O O O HOO HOON +C l C H 3C H 3C H 3H OFormation of the catHEC Schematic diagram of adsorption of catHEC onto silica surface. (1) CatHEC No. HEC (g) Glycidyltrimethylamminium chloride (g) Viscosity () Nitrogen content (%) CatHEC1 380 CatHEC2 375 CatHEC3 370 Preparation and properties of CatHEC 0 2 4 6 8 10pH= 2. 2M igr at ion tim e (m in)123(a)0 2 4 6 8 10 12 14pH=M igration time (min)123(b)0 2 4 6 8 10 12 14pH=M igration time (min)123(c)2 4 6 8 10 12 14pH=M igration time (min)123(d)2 4 6 8 10864202468μeof (108m2V1s1)pH Bare capillary HEC Cat HEC 1 Cat HEC 2 Cat HEC 30 2 4 6 8 10 12 14 CatHEC2 coatingBare capillary M igration time (min)123HEC coatingElectroosmotic flow as a function of pH. Comparison between a bare fusedsilica capillary, HEC and catHEC coated capillary Electropherograms of a mixture of standard proteins. Separations were carried out using catHEC2 coated capillary 1 = Lysozyme。 3, ribonuclease. (A) Typical TEM images of (a) PEO113bP4VP45, (b) PEO113bP4VP90, (c) PEO113bP4VP113, and (d) PEO113bP4VP294。 Tf, transferrin。 (c) 4% w/v。 2=Lysoz
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