科大高分子課件四聚合物在生物高分子分離中的應(yīng)用-全文預(yù)覽
【正文】 12 14 16 18 20 22 24 26 28 30 3212 14 16 18 20 22 24 26 28 30 3212 14 16 18 20 22 24 26 28 30 32d Mi g r a ti o n ti m e ( m in)c1353107887260331028127123419411872 b Intensity aSeparation of Φ 174/HaeIII digest by CE with quasiIPN3 at (a) 2% w/v。 IgG, immunoglobulin G. Electrophoresis 2022, 29, 28122819 3. 多功能分離介質(zhì)的合成及應(yīng)用研究 (1) HECgPDMA 研究背景 能進(jìn)行 DNA、蛋白質(zhì)、氨基酸、同分異構(gòu)體等的分離 14 15 16 17 18 19 20 21 22 23 24 25 26 2713 14 15 16 17 18 19 20 21 22 23 24 25 2612 13 14 15 16 17 18 19 20 21 22 23 24 2510782343102711941181353281872603 M i g r a t i o n t i me ( mi n )c721353107887260331028127123419472118 Intensityb13531078872603281271310234194118 a+Separation of Φ 174/HaeIII digest by CE with HECgPDMA at (a) % w/v。 α2M, α2macroglobulin。 500V/cm。 3 = Ribonuclease A. ２．結(jié)構(gòu)規(guī)整的聚合物 PEObP4VP N O O B r O O O O N N + B r samples Mn 1H NMRa) ( 104) Mn， GPCb) ( 104) Mw/Mn (GPC) PEO113bP4VP45 PEO113bP4VP90 PEO113bP4VP113 PEO113bP4VP294 a. Mn of PEObP4VP estimated by 1H NMR； b. Mn of PEObP4VP determined by GPC. Formation of the PEObP4VP 1H NMR and GPC data of PEObP4VP copolymers Effect of molecular weight of P4VP block on the separation of basic proteins at pH . 1, lysozyme。 3 = Ribonuclease A. Electropherograms of a mixture of standard proteins at pH . 0 2 4 6 8 10 12M igration time (min)PH=first runfiftieth run(a)1230 2 4 6 8 10 12 14M igration time ( min)first runfiftieth runpH=(b) 1230 2 4 6 8M igration time (min)first runfiftieth runpH=(c)123Protein Bare capillary N (plate/m) b HEC N (plate/m) RSD (%) Cat
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