【正文】 堿代謝的調(diào)節(jié)作用［ J］海洋學報， （ 2）： 117— 123； [10] 邊猛 海洋真菌產(chǎn)生生物堿代謝調(diào)節(jié)的研究［ D］：［碩士學位論文］青島科技大學 生物化工系 。t charge you more than 35% of your pensation if you win the case. you39。s actions. CSKA said they were surprised and disappointed by Toure39。t really grow up, it seems is not so important。也曾經(jīng)好像已經(jīng)下定了決心去做某件事，但更多的時候是最后又打起了退堂鼓。突然想到那件藍格子襯衫 ；那些被折成各種各樣形狀的信紙；那段從街角深巷伊始的友誼；還有那場還沒有開始就宣告了終結的邂逅計劃 …… 那些年那些天的非做不可，終于和青春一樣，都將在我們的人生中謝幕。屏住呼吸，生活停滯；呼出呼吸，更多新奇與經(jīng)歷又將拉開序幕。ll be traveling fast, so despite the distance, it will not be like diving into the deep end of a pool. It will be like he is diving into the shallow end. Skydiver preps for the big jump When he jumps, he is expected to reach the speed of sound 690 mph (1,110 kph) in less than 40 seconds. Like hitting the top of the water, he will begin to slow as he approaches the more dense air closer to Earth. But this will not be enough to stop him pletely. If he goes too fast or spins out of control, he has a stabilization parachute that can be deployed to slow him down. His team hopes it39。與其一味顧慮重重，不如從局外人的角度看待決策之事。 Whenever a painful situation arises in your life, try to embrace it instead of running away or trying to mask the hurt. When the sadness strikes, take a deep breath and lean into it. When we run away from sadness that’s unfolding in our lives, it gets stronger and more real. We take an emotion that’s fleeting and make it a solid event, instead of something that passes through us. 當生活中出現(xiàn)痛苦的事情時，別再逃跑或隱藏痛苦，試著擁抱它吧；當悲傷來襲時，試著深呼吸，然后直面它。 Have declared the end of the encounter that haven39。s time, your ideas are always special to clear. Want, want, line is clear, as if nothing could shake his. Also once seemed to be determined to do something, but more often is he backed out at last. Dislike his cowardice, finally found that there are a lot of love, there are a lot of miss, like shadow really have been doomed. Those who do, just green years oneself give oneself an arm injection, or is a selfrighteous spiritual. 一個人的時候，自己的想法總是特別地清晰。 The end of our life, and can meet many things really do? 而窮盡我們的一生，又能遇到多少事情是真正地非做不可？ During my childhood, think lucky money and new clothes are necessary for New Year, but as the advance of the age, will be more and more found that those things are optional。s 21 win. Michel Platini, president of European football39。t 11 agree with the disciplinary action your employer has taken against you your employer dismisses you and you think that you have been dismissed unfairly. For more informu, take advice from one of the anisations listed under Further help. Employment tribunals are less formal than some other courts, but it is still a legal process and you will need to give evidence under an oath or affirmation. Most people find making a claim to an employment tribunal challenging. If you are thinking about making a claim to an employment tribunal, you should get help straight away from one of the anisations listed under Further help. ation about dismissal and unfair dismissal, see Dismissal. You can make a claim to an employment tribunal, even if you haven39。inactivation or deletion of phoP reduces the negative phosphate regulation of pimaricin biosynthesis metabolic engineering 2022 ,9 (2)： 217— 227； [2] PEDERSEN J C Natamycin as a fungicide imager media ［ J］ Applied and environment 。 納他霉素是和乳酸鏈球菌素并列的僅有的兩種生物防腐劑，其具有毒性低且不會造成食品風味巨大改變的特性，是一種極具有使用、生產(chǎn)價值的抗生素。 01CK 1% 2% 5% 8% 10% 15% 20%納他霉素產(chǎn)量（g因此，產(chǎn)黃青霉的最佳培養(yǎng)時間為 1d。當在發(fā)酵培養(yǎng) 12h 時添加效果最佳，此時菌株納他霉素產(chǎn)量達 ，較對照提高 ％。 2．結果及分析 納他霉素標準曲線的制作 以納他霉素濃度為橫坐標， OD303 值為縱坐標繪制納他霉素標準曲線，見圖 1。確定真菌發(fā)酵液最適添加時間。 培養(yǎng)基 種子培養(yǎng)基 (g/L)：葡萄糖 酵母浸膏 蛋白胨 氯化鈉 10， pH 發(fā)酵培養(yǎng)基 (g/L)：葡萄糖 酵母浸膏 大豆蛋白胨 淀粉 40， 實驗方法 納塔爾鏈霉菌的培養(yǎng) 用接種環(huán)刮取斜面菌種一環(huán)于裝有 25mL 種子培養(yǎng)基的 250mL 三角瓶中， 28℃、 200r/min 搖床培養(yǎng)， 40h 后以 9%接種量 接種于裝有 30mL 發(fā)酵培養(yǎng)基的 250mL 三角瓶中，28℃、 200r/min 搖床培養(yǎng) 4d。 由于各種因素的制約，國內(nèi)納他霉素的主要來源至今仍然是從國外進口。 關鍵詞：納塔爾鏈霉菌 N5 菌株 納他霉素 產(chǎn)黃青霉 黑曲霉 釀酒酵母 前言 1955 年 struyk 等人首次從南非 natal 州土壤中分離得到納塔爾鏈霉菌（ streptomyces natalensis），并發(fā)現(xiàn)該菌種可以產(chǎn)生一種內(nèi)酯類抗生素， strnyk 將其命名為匹馬菌素（ pimaricin） [1]， 后世界衛(wèi)生組織將其統(tǒng)一命名為納他霉素。 合肥 二 o 1 3 年 5 月 目 錄 摘要 1 前言 1 1 實驗材料及方法 2 實驗材料 2 實驗菌種 2 培養(yǎng)基 2 實驗方法