【正文】 s most popular dishes are his brainchild. Lin says his secret of success is innovation rooted in experience: He spends months every year traveling and eating worldwide, seeking new ingredients and inspirations that could improve the offerings of Blue Frog restaurants. The restaurant chain regularly launches a new seasonal menu, usually featuring eight new dishes with fresh ingredients. The bestselling seasonal dishes later replace the least popular items on the regular menu, which is usually updated in late fall and boasts a selection of about 40 dishes. Food should have no boundaries, Lin says. As long as the food tastes good and is healthy, it doesn39。t conducive to raising a child. She planned to have children once she had achieved career success so she could provide a stable environment for her baby. After 10 years, Xiao felt the time was right, and she tried to conceive for about six months, but was unable to bee pregnant. I conducted prehensive physical checkups. The results suggested that Xiao39。s digitized society, will also be attached on the report.Myopia is not only a disease that makes people see things blurrily, but also leads to severeplications, such as glaua (increased pressure within the eyeball), and can causeblindness, said Zhou Yuehua, an established eye care specialist with Beijing TongrenHospital.It is very important for parents and children to know about the risk and care for their eyes.There are about 450 million myopia patients in China. Among Chinese myopia patients,30million are severe patients, according to ZhouThe prevalence of myopia among high school and college students is more than 70 percent,and the situation is continuously worsening, he Zhu, director with the China Youth Development Service Center, said long hours ofstudy, lack of exercise, especially outdoor activities, and attachment to electronic screen aresome of the top reasons behind the widespread myopia problems, and the increase ofyounger patients, although China has made great progress in improving the hardwarefacilities and lighting conditions in said he hopes the survey will alert people on the threatening situation, and also helpmedical experts to better guideline parents and children39。還有教過我的所有老師 們，你們嚴(yán)謹(jǐn)細(xì)致、一絲不茍的作風(fēng)一直是我工作、學(xué)習(xí)中的榜樣； 老師們 循循善誘的教導(dǎo)和不拘一格的思路給予我無(wú)盡的啟迪在此謹(jǐn)向 所有教過我的 老師致以誠(chéng)摯的謝意和崇高的敬意。 研究表明 ， 植物 在 耐旱性、 抗寒性和耐鹽性 環(huán)境條件下對(duì)植物次生代謝產(chǎn)物的合成與積累 具有重要作用 [20]， 合成 植物 次生代謝產(chǎn)物的種類及數(shù)量是 根據(jù)所處的環(huán)境條件及其變化來(lái)決定 [21]。在良好的 生長(zhǎng)環(huán)境中高等植物葉綠體具有結(jié)構(gòu)完整、層次清晰、排列整齊等特點(diǎn)， 但在 高溫 [17]和 鹽脅迫 [18]下 ，植物 葉綠素含量降低 是由于植物細(xì)胞中色素系統(tǒng)遭到破壞所 導(dǎo)致 的 。 表 5 標(biāo)準(zhǔn)曲線的繪制 圖 蛻皮激 素濃度 (mg** 處理 (mmolSD， n=5) 由圖 8 可知，隨著鹽濃度的升高，露水草中丙二醛含量也在逐漸增加，且各實(shí)驗(yàn)組與對(duì)照組都存在顯著差異，但是鹽濃度為 50、 70、 90mmolL1，70 nmol** 120 177。** 70 177。FW1) CK 177。L1) 葉綠素 a 葉綠素 b 葉綠素 a+葉綠素 b 類胡 蘿卜素 (mgL1時(shí)實(shí)驗(yàn)組與對(duì)照組比較時(shí)差異最大，且實(shí)驗(yàn)組的葉綠素 a 占對(duì)照組的 %， 對(duì)于葉綠素 b 鹽濃度為 120 mmol.002** 177。+ 177。 177。s 1） /（ mmolL1時(shí)，實(shí)驗(yàn)組的凈光合速率 占 對(duì)照組的 %， 實(shí)驗(yàn)組的蒸騰速率 占 對(duì)照組 的 %，實(shí)驗(yàn)組的 氣孔導(dǎo) 度 占對(duì)照組的 %，而對(duì)于胞間二氧化碳影響最大的鹽濃度是 120 mmol樣品蛻皮激素含量（ mgg1 丙二醛 的測(cè)定 丙二醛 的提取 ： 稱 露水草 葉片 ， 加入 2mL10%TCA 研磨 及少量石英砂 ，紅河學(xué)院本科畢業(yè)論文 (設(shè)計(jì) ) 5 研 磨至 勻漿 ， 再加 4mL10%TCA 進(jìn)一步研磨 ， 勻漿 置于離心管中 ， 以 4000r樣品鮮重 （ 5） 相對(duì)電導(dǎo)率 的測(cè)定 先用蒸餾水清洗帶塞子的玻璃試管 2 次，再用去離子水浸泡過夜，最后倒置晾干。s1，飽和二氧化碳設(shè)為 500ppm） ， 當(dāng)光合速率穩(wěn)定且胞間 二氧化碳大于零時(shí) 可以保存此次記錄， 測(cè)定完一個(gè)數(shù)據(jù)時(shí)剪下測(cè)定部位的葉面積，并材料與方法 4 坐標(biāo)紙上畫上葉面積； ④ 數(shù)據(jù)存取。 紅河學(xué)院本科畢業(yè)論文 (設(shè)計(jì) ) 3 實(shí)驗(yàn)材料，儀器及試劑 實(shí)驗(yàn)材料 材料 ： 露水草種子 購(gòu)于葆元種業(yè) 。因此 ， 環(huán)境脅迫有利于或促進(jìn)藥用植物次生代謝有效成分的積累。 土壤鹽漬化是現(xiàn)代農(nóng)業(yè)所面臨的主要問題之一，鹽分脅迫影響著植物產(chǎn)量、蛋白質(zhì)合成和光合作用以及能量代謝 [11]。 傳統(tǒng)藥用價(jià)值主要體現(xiàn)為祛風(fēng)活絡(luò)，利濕消腫，退虛熱。 physiological indicators。紅河學(xué)院本科畢業(yè)論文 (設(shè)計(jì) ) I 2021 年度本科生畢業(yè)論文（設(shè)計(jì)） 鹽脅迫對(duì)露水草生理和有效成分含量的影響 院 － 系： 生命科學(xué)與技術(shù)學(xué)院 專 業(yè)： 生物技術(shù) 年 級(jí)： 2021 學(xué)生姓名： 學(xué) 號(hào)： 導(dǎo)師及職稱： （講師） 2021 年 4 月 紅河學(xué)院本科畢業(yè)論文 (設(shè)計(jì) ) II 2021 Annual Graduation Thesis (Project) of the College Undergraduate The Effect on The Physics and Proportion of Cyanotis arachnoidea by Salt Stress Department: College of Life Science and Technology Major: Biotechnology Grade: 2021 Student’s Name: Student No.: 202101130277 Tutor: Lecturer Tao HongZheng April, 2021 紅河學(xué)院本科畢業(yè)論文 (設(shè)計(jì) ) III 摘 要 本研究利用不同濃度的 中性 鹽溶液 處理露水草幼苗，并測(cè)定在不同濃度的鹽脅迫下露水草的各項(xiàng)生理指標(biāo)和次生代謝產(chǎn)物含量的變化 ，從而研究出最適合促進(jìn)露水草次生代謝產(chǎn)物含量 增加的鹽濃度。 βecdysone 紅河學(xué)院本科畢業(yè)論文 (設(shè)計(jì) ) V 目 錄 ............................................................................................................................. 1 露水草的研究現(xiàn)狀 .................................................................................................. 1 鹽脅迫的研究現(xiàn)狀 .................................................................................................. 1 ................................................................................................................. 3 實(shí)驗(yàn)材料，儀器及試劑 .......................................................................................... 3 實(shí)驗(yàn)方法 .................................................................................................................. 3 ................................................................................................................. 6 鹽脅迫對(duì)露水草光合參數(shù)的影響 .......................................................................... 6 鹽脅迫對(duì)露水草的光合色素影響 .......................................................................... 8 鹽脅迫對(duì)露水 草的相對(duì)電導(dǎo)率影響 ...................................................................... 9 鹽脅迫對(duì)露水草中丙二醛含量的影響 ................................................................ 10 鹽脅迫對(duì)露水草的蛻皮激素含量的影響 ............................................................ 11 ..............................................