【正文】 = 15 , respectively, the mice were killed at 5,7,9 weeks, every five mice in each group were tissue embedded, sliced, microscopic examination. By microscopic examination of the spleen cell morphology, using an enzymelinked immunoassay serum IgG levels to explore joint copper molybdenum on immune function in mice. The pathological results showed a clear structure of the control group of mice spleen cells arranged in neat rows, while the other three groups of condensation nuclei or deposition, severe structural damage, a large number of vacuoles, spleen lymphocytes , when pared with the control group five weeks, in addition to differences in blood levels of immunoglobulin in the four groups of mice significant difference between five and seven weeks when the mice were not significant using the mouse immunoglobulin enzyme II has nine weeks, when pared with the control group increased immunoglobulin levels, other groups were joint can cause pathological damage to the mouse spleen, the spleen index significantly Copper Federation reduce immunity in mice, but up to a certain extent, copper decreased immune function delay. KEY WORDS: mice ， spleen ， molybdenumcopper ， immune function ，immunoglobulin III 目 錄 1 前言 ............................................................................................................................... 1 鉬 .................................................................................................................................. 1 銅 .................................................................................................................................. 2 小鼠的免疫系統(tǒng) ........................................................................................................... 2 脾臟 ........................................................................................................................... 2 2 材料與方法 ................................................................................................................. 4 主要儀器 及設(shè)備 .......................................................................................................... 4 主要試劑 ...................................................................................................................... 4 化學藥品 ................................................................................................................... 4 小鼠免疫球蛋白 IgG 酶聯(lián)免疫分析試劑盒 .......................................................... 5 統(tǒng)計學處理 ............................................................................................................... 5 主要藥品及試劑的配制 .............................................................................................. 5 HE 染色相關(guān)試劑 ..................................................................................................... 5 試驗動物 ...................................................................................................................... 5 動物分組與處理 .......................................................................................................... 5 動物組織病理切片的制作 .......................................................................................... 6 取材與修塊 ............................................................................................................... 6 固定 ........................................................................................................................... 6 沖洗 ........................................................................................................................... 6 脫水 ........................................................................................................................... 6 透明 ........................................................................................................................... 6 浸蠟 ........................................................................................................................... 6 包埋 ........................................................................................................................... 6 石蠟塊的整修 ........................................................................................................... 7 切片與展片 ............................................................................................................... 7 貼片及烤片 ............................................................................................................. 7 HE 染色 ........................................................................................................................ 7 酶聯(lián)免疫分析 .............................................................................................................. 7 小鼠免疫球蛋白 IgG 酶聯(lián)免疫分析 ...................................................................... 8 3 結(jié)果 ............................................................................................................................... 9 脾臟的形態(tài)學變化 ...................................................................................................... 9 小鼠的臨床表現(xiàn) ........................................................................................................ 10 鉬銅聯(lián)合對小鼠免疫學指標的影響 .........................................................................11 IV 4 分析與討論 ............................................................................................................... 12 鉬銅聯(lián)合對小鼠脾臟的形態(tài)學影響 ........................................................................ 12 鉬銅對小鼠免疫學指標的影響 ................................................................................ 13 鉬銅對小鼠血清中 IgG 含量的影響 .................................................................... 13 5 結(jié)論 ............................................................................................................................. 14 參考文獻 ..............................................................................................................