【正文】
植物是地高辛的唯一來源。(一)試劑配制磷酸緩沖液PBS():磷酸鈉鹽 50mM,NaCl 200mM。TdT酶反應(yīng)液:TdT酶32μl;TdT酶緩沖液 76μl,混勻,置于冰上備用。過氧化物酶標(biāo)記的抗地高辛抗體(ONCOR)(二)實(shí)驗(yàn)步驟標(biāo)本預(yù)處理:(1)石蠟包埋的組織切片預(yù)處理:將組織切片置于染色缸中,用二甲苯洗兩次,每次5min。用蒸餾水洗4次,每次2min,然后按下述步驟2進(jìn)行操作。用PBS洗兩次,每次5min,然后按下述步驟2進(jìn)行操作。色缸中加入含2%過氧化氫的PBS,于室溫反應(yīng)5min。將切片置于染色缸中,加入已預(yù)熱到37℃的洗滌與終止反應(yīng)緩沖液,于37℃保溫30min,每10min將載玻片輕輕提起和放下一次,使液體輕微攪動(dòng)。用蒸餾水洗4次,前3次每次1min,最后1次5min。1 用二甲苯脫水3次,每次2min,封片、干燥后,在光學(xué)顯微鏡下觀察并記錄實(shí)驗(yàn)結(jié)果。TUNEL assayTerminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the terminal end of nucleic acids.TUNEL is a mon metho