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本科畢業(yè)論文 題 目 : 不同生長期異堇葉碎米薺葉 揮發(fā)性成分及硒含量分析 ID:071040128 BACHELOR39。S THESIS OF HUBEI UNIVERSITY FOR NATIONALTIES Analyse the volatile ponents and selenium contents of Cardamine violifolia at different growth periods Name: Di Mou Grade: 0710401 Profession: Biological engineering Tutor: Zhou Enshi ? China May, 20xx 學(xué)術(shù)聲明 堅持以 “求實、創(chuàng)新 ”的 科研 精神從事研究工作。 本論文中除引文外,數(shù)據(jù)和有關(guān)材料均 是真實的。 其他同志對本研究所做的貢獻(xiàn)已在論文中做了聲明并表示了謝意。 本聲明的法律后果由本人承擔(dān)。 作者簽名: 年 月 日 導(dǎo) 師 審 核 聲 明 本人 鄭重 聲明:該 生上交 的 學(xué)士 學(xué)位論文,是在本人的指導(dǎo)下獨立進(jìn)行研究所取得的成果。其內(nèi)容均經(jīng)本人審核,同意作為該 生 的學(xué)位論文提交。 導(dǎo)師簽名: 年 月 日 不同生長期異堇葉碎 米薺葉揮發(fā)性成分及硒含量分析牟 迪 (湖北民族學(xué)院生物科學(xué)與技術(shù)學(xué)院,湖北恩施, 445000) 摘 要 : 為了探尋異堇葉碎米薺葉片不同生長期揮發(fā)性成分和硒含量,主要通過頂空固相微萃取氣相色譜 質(zhì)譜聯(lián)用儀( SPMEGC/MS)測定揮發(fā)性成分,通過石墨消解儀將葉片消解后,用雙道原子熒光光度計測定出硒含量。結(jié)果表明: 1)異堇葉碎米薺葉片揮發(fā)性物質(zhì)主要為異硫氰酸芐酯、 2,2,4,6,6五甲基庚烷、苯乙腈,其相對含量在各個時期中較高; 2)異硫氰酸芐酯在不同時期異堇葉碎米薺葉片中相對含量 較 高,在幼苗期高達(dá) %; 3)隨著異堇葉碎米薺的生長葉片硒含量呈顯著性增加,到開花期 時, 達(dá)到最高,隨后變化不顯著,幼苗期硒含量最低為 Kg1,花期硒含量最高為 mgKg1。 關(guān)鍵詞 : 異堇葉碎米薺;揮發(fā)性成分;硒 Analyse the volatile ponents and selenium contents of Cardamine violifolia at different growth periods Di Mou (College of Biological Scientific and technical,Hubei Minzu University , Hubei Enshi, 445000, ) Abstract:In order to explore the volatile ponents and selenium content of Cardamine violifolia at different growth periods, we measure the volatile ponents by using SPMEGCMS and determine the selenium contents by digesting with Graphite digestion apparatus and detecting with double channel atomic fluorescence spectrometer. It turn out that: 1)the main volatile substances which relative contents are higher in every periods of Cardamine violifolia are Benzyl mustard oil、2,2,4,6,6Pentamethylheptane and Benzyl cyanide。2)Benzyl mustard oil is the highest substance in Cardamine violifolia at different growth periods, which reach to the maximum of % at seeding stage。3)As the Cardamine violifolia grows older,the more selenium contents are accumulated, and the content e to the highest at flowering stage, but the change is not significant afterwards. At the seeding stage ,selenium contents are in a minimum of Kg1 ,while the selenium contents are the highest during flowering stage of mgKg1. Key words: Cardamine violifolia; volatile substances; selenium 目錄 1 文獻(xiàn)綜述 ............................................................................................ 1 硒的性質(zhì)及藥用價值 .........................................................................................1 硒的性質(zhì) ....................................................................................................1 硒的藥用價值 ............................................................................................1 抗癌 ..................................................................................................1 抗氧化 ..............................................................................................2 解毒作用 ..........................................................................................2 硒的其他作用 ..................................................................................2 植物富硒 .............................................................................................................2 植物富硒 能力的劃分 ................................................................................2 植物對硒的吸收和代謝 ............................................................................3 異堇葉碎米薺的性質(zhì) ..........................................................................................4 異堇葉碎米薺特征 ....................................................................................4 異堇葉碎米薺的地理分布 ........................................................................5 異堇葉碎米薺的研究現(xiàn)狀 ........................................................................5 選題意義 ..............................................................................................................6 2 材料與方法 .....................................................