對(duì)羧基苯硼酸-明膠納米微球制備及抗腫瘤療效研究-本科畢業(yè)論(已修改)
2025-06-21 18:53 本頁面
【正文】 本科畢業(yè)論文（設(shè)計(jì)、創(chuàng)作） 題 目： 對(duì)羧基苯硼酸 明膠納米微球制備及抗腫瘤療效研究 題目類別： □√ 論 文 □設(shè) 計(jì) □ 創(chuàng) 作 學(xué)生姓名： XXX 學(xué)號(hào)： XXXXXXX 所在院系： 生命科學(xué)學(xué)院 專業(yè)： 生物技術(shù) 入學(xué)時(shí)間： 2021 年 9 月 導(dǎo)師姓名： 王鑫 職稱 /學(xué)位： 教授 導(dǎo)師所在單位： 安徽大學(xué)生命科學(xué)學(xué)院 完成時(shí)間： 2021 年 6 月 安徽大學(xué)教務(wù)處 制 對(duì)羧基苯硼酸 明膠納米微球制備及抗腫瘤療效研究 摘 要 目標(biāo)： 以 明膠 為起始材料制備納米微球，并在其表面修飾具有腫瘤靶向功能的小分子配體對(duì)羧基苯硼酸。方法：利用不良溶劑法制備不同明膠納米微球，利用對(duì)羧基苯硼酸的羧基與明膠微球表面的氨基形成酰胺鍵制備靶向納米微球。利用動(dòng)態(tài)光散射研究了納米微球的粒徑分布以及在不同生理?xiàng)l件下的穩(wěn)定性。 利用動(dòng)態(tài)光散射納米激光粒度儀測(cè)量了納米微 球在不同 pH 值下的表面電荷變化。 利用 透射電子顯微鏡 來 察看了納米微球 的表 形 特征 。負(fù)載抗腫瘤藥物阿霉素，并 探 究了載藥納米微球的體外釋放行為。 結(jié)果：制備的明膠納米微球及靶向納米微球粒徑在 160 nm 左右 。穩(wěn)定性實(shí)驗(yàn) 分析數(shù)據(jù) 表明納米微球在不同生理環(huán)境下都具有良好的穩(wěn)定性。體外藥物釋放實(shí)驗(yàn)證明負(fù)載阿霉素的納米微球可以順利的將藥物釋放出來，且釋放行為具有一定的 pH 依賴性。細(xì)胞毒性實(shí)驗(yàn)證明載藥納米微球可以很好的抑制人結(jié)腸癌細(xì)胞的生長。 關(guān)鍵詞： 對(duì) 羧基苯硼酸；明膠；納米微球；抗腫瘤 4Carboxyphenylboronic acid gelatin nanoparticles preparation and antitumor efficacy research Abstract Objective: Gelatin was chosen as an initial material to prepare nanoparticles. 4Carboxyphenylboronic acid (4CPBA) was then modified on the surface of gelatin nanoparticles to give tumortargeting nanoparticle drug delivery systems. Methods: The size and distribution of these two nanoparticles were measured by dynamic light scattering (DLS). The micromorphology of nanoparticles was observed by transmission electronic microscope (TEM). Zeta potential of gelatin nanoparticles and 4CPBA modified nanoparticles was measured by DLS. The stability of these two nanoparticles in different conditions were then investigated. Drug release from nanoparticles were measured at pH , and . Cytotoxicity of these two nanoparticles were evaluated against HCT cell line. Results: TEM images display that gelatin nanoparticles and 4CPBA modified nanoparticles are spherical like with a uniform diameter around 160 nm. Both tow nanoparticles display an extraordinary stability in different conditions. DOX was successfully loaded into NP1 and NP2, and DOX release rate from nanoparticles increase significantly with the change of pH value from to . The results of MTT assay demonstrated that these DOXloaded nanoparticles can efficiently inhibit the growth of HCT cells. Key words: 4Carboxyphenylboronic acid。 Gelatin。 nanoparticles。 antitumor 目 錄 1 引言 ......................................................................................................................................... 1 明膠的基本概述 ............................................................................................................. 1 載藥納米微球的基本概述 .............................................................................................. 1 腫瘤治療的基本概述 ...................................................................................................... 2 論文選題意義 ................................................................................................................ 2
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