菊花嫩莖快繁技術的研究生物技術及應用-文庫吧
2025-03-20 23:51 本頁面
【正文】 答辯小組長簽字 年 月 日菊花嫩莖快繁技術的研究06生物技術及應用（2）班 吳黎平 指導老師：黃小忠摘要：先實驗前的準備工作，然后選取菊花，以帶頂芽的嫩莖為外植體接種到誘導培養(yǎng)基中，放在培養(yǎng)室里培養(yǎng)并觀察，一周后長出叢生芽，再沒有污染的叢生芽在無菌超凈工作臺上用手術刀切成多塊，再將叢生芽接到繼代培養(yǎng)基中進行繼代培養(yǎng)，當發(fā)現初步長出嫩根時，將無污染的帶根的叢生芽接到生根培養(yǎng)基中，當生長成幼苗時將生根試管苗開蓋練苗,移栽。及在培養(yǎng)過程中遇到的問題。關鍵詞：菊花；組織培養(yǎng)；褐變；脫毒；外植體Rapid Propagation of Chrysanthemum tender stem ResearchAbstract: Tests before first the preparatory work, then the selection chrysanthemum, plants the body take the belt terminal bud tender stem as outside to vaccinate in the induction culture medium, places in the raise room to raise and to observe, after a week is long grows thickly the bud, then does not have the pollution to grow thickly the bud to sliver the multiblocks on the asepsis ultra only work table with the scalpel, then will grow thickly the bud grafting to continue in a generation of culture medium to carry on continues a generation of raise, when discovered when at the beginning of the length of stride will leave the tender root, will not have the pollution the belt root to grow thickly the bud grafting to take root in the culture medium, when will grow the seedling will take root the test tube seedling to uncap practices the seedling, will transplant. And question which meets in the raise process.Keyword：Chrysanthemum。 Tissue culture。 Turning brown。 Escapes the poison。 Outside plants the body目 錄1．材料和方法………………………………………………………………81．1材料與用具……………………………………………………………81．2培養(yǎng)條件……………………………………………………………… 91．3實驗前的準備工作…………………………………………………… 91．3．1培養(yǎng)基配制………………………………………………………… 91．3．2接種室的滅菌……………………………………………………… 91．3．3超凈工作臺及器械消毒…………………………………………… 91．3．4材料選取…………………………………………………………… 91．3．5材料滅菌…………………………………………………………… 91．4接種…………………………………………………………………… 101．5誘導側芽生長……………………………………………………………101．6 繼代培養(yǎng)……………………………………………………………… 101．7 誘導生根………………………………………………………………101．8 移栽培養(yǎng)………………………………………………………………102結果與分析…………………………………………………………………10 初代培養(yǎng)…………………………
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