20xx探討在?高海拔地區(qū)應(yīng)用血液基因組dna提取試劑盒的最佳條件精選-文庫(kù)吧
2025-03-30 05:18 本頁(yè)面
【正文】 re between 20 and 50 ng/mu。L, 35 were between 5180 ng/mu。L, and 61 samples were80 ng/mu。 concentration of DNA was higher than the control group （Plt。） .The ratio of the DNA purity OD260/OD280 in the experimental group 109 samples was 105 in the sample, and the ratio was less than ratio was less than , and the ratio was greater than that of the control group （Plt。） .Conclusion Uses reagent box extraction blood genome team DNA in the high elevation area, needs to optimize the extraction condition, obtains the DNA density and the purity can satisfy the downstream experiment to request, also the duplication is good. Keyword:plateau environment。 DNA extraction。 optimization?！ ∪蚪MDNA是科學(xué)研究、臨床疾病分析及親子鑒定等許多研究領(lǐng)域不可缺少的原材料。目前， 提取DNA的各類方法較多， 除各實(shí)驗(yàn)室有本身獨(dú)特的方法外， 應(yīng)用最為廣泛的還屬商品化的血液基因組提取試劑盒， 不僅使用方便本錢低廉且液體量較少相對(duì)污染較低。西藏自治區(qū)地處我國(guó)西南邊疆， 平均海拔在3000米以上， 由于地勢(shì)高而構(gòu)成了特有的高原低壓低氧、酷寒單調(diào)的環(huán)境。在高原環(huán)境中提取血液基因組可采納商品化的血液基因組提取試劑盒， 但是假設(shè)按照試劑盒中說(shuō)明書方法進(jìn)接提取血液基因組其產(chǎn)量和純度特別難滿足后續(xù)研究的需要。本課題組通過(guò)屢次嘗試研究， 探究出了一套血液基因組試劑盒提取DNA的優(yōu)化方法， 該方法在高原環(huán)境下提取的DN
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