【正文】
重復次數最多的是重復5次,有53條SSR,%;其次是重復次數為6次的,有36條SSR,占30%;其次是7次和8次的,而重復次數為9次和22次的為最少,只有1條SSR。并且SSR的重復次數都在5以上,由此可以推測利用鵪鶉核苷酸序列和EST序列中的SSR序列將有可能開發(fā)一批有價值的SSR分子標記供各種研究所用。盡管SSR具有廣泛的用途,但是其開發(fā)過程卻是相當費時費力,目前SSR標記的開發(fā)有4種方法:文庫篩選法,基于錨定PCR技術的方法,生物信息學方法,轉移擴增法。Hayden 和Sharp [11]在錨定PCR的基礎上發(fā)明了SAM法。參考文獻:[1] Kayang B B, Vignal A, InoueMurayama M A et al. Firstgeneration microsatellite linkage map of the Japanese quail. Anim Genet. 2004, 35(3): 195200.[2] Kayang B B, Vignal A, InoueMurayama M A et al. Integrated maps in quail (Coturnix japonica) confirm the high degree of synteny conservation with chicken (Gallus gallus) despite 35 million years of divergence. BMC Genomics, 2006, 7:118.[3] Fisher P J, Richardson T E, Gardner R C, et al. Characteristics of single and multicopy microsatellites from Pinus radiata. Theor Appl Genet, 1998, 96: 969-979[4] Varghese J P, Rudolph B, Uzunova M I, et al. Use of 518