【正文】 % of CD4+ CD25+ cells in CD4+ cells PBLs SPL LNs a c b NP THYnude mice Control Balb/c mice NP THYnude mice Control Balb/c mice B A Nude mice Balb/c mice NP THYnude mice CD25 CD4 The presence of mouse CD4+CD25+Treg cells in NP THYgrafted nude mice Sun ZY, Wang HJ, Sun LG, Zhao L, Yi HF, Li QH, Peng JX, and Zhao Y. American J of Transplantation 2022， 6： 110. A Cell number FOXP3 89% 91% NP THYnude mice Balb/c mice CD4+CD25+T cells CD4+CD25 T cells B C % of FOXP3+ cells/CD4+ CD25+ cells 0 10 20 30 40 50 60 70 80 90 100 NP THYnude mice Control Balb/c mice MFI of FOXP3+ T cells 0 10 20 30 40 50 60 NP THYnude mice Control Balb/c mice Normal expression of Foxp3 in CD4+CD25+Treg cells in NP THYgrafted nude mice Sun ZY, Wang HJ, Sun LG, Zhao L, Yi HF, Li QH, Peng JX, and Zhao Y. American J of Transplantation 2022， 6： 110. AlloMLR Response to OVA A B [3 H]Thymidine uptake (cpm x 103) 0 2 4 6 8 10 12 14 Treg cells from NP THYnude mice Treg cells from Balb/c mice * 0:1 1:1 1:2 1:4 1:8 *** *** ** ** Treg: T responder cells 0 5 10 15 20 25 30 0:1 1:1 1:2 1:4 1:8 1:16 1:32 [3 H]Thymidine uptake (cpm x 103) Treg: T responder cells *** *** *** ** *** *** * The function of Treg cells in NP THYgrafted nude mice Sun ZY, Wang HJ, Sun LG, Zhao L, Yi HF, Li QH, Peng JX, and Zhao Y. American J of Transplantation 2022， 6： 110. 10 12 14 16 18 20 22 24 26 28 1 2 3 4 5 6 7 8 9 10 11 Body weight (g) Weeks Treg cells from NP THYnude mice + Tresp cells (1:2) Tresp cells (CD4+CD25T cells) Treg cells from Balb/c mice + Tresp cells (1:2) Treg cells from Balb/c cells + Tresp cells (1:1) Treg cells from NP THYnude mice + Tresp cells (1:1) Control mice received no cells * * * * ** ** ** ** ** ** ** ** ** ** ** ** The function of Treg cells in NP THYgrafted nude mice Sun ZY, Wang HJ, Sun LG, Zhao L, Yi HF, Li QH, Peng JX, and Zhao Y. American J of Transplantation 2022， 6： 110. 人 T細(xì)胞可以在異種豬胸腺中可以進(jìn)行正常分化嗎 ? Phenotype and repopulation of T cell from grafts of FH Thy/Livgrafted SCID mice Zhang Baojun et al, unpublished data FH THY NP THY/FH LIV The levels of human CD4+ T cells in the peripheral blood of FH Thy/Livgrafted SCID mice AntihTCRFITC AntihCD8PE AntihCD4PE Zhang Baojun et al, unpublished data Exp Control Exp Control Tolerance to donor pig of human T cells maturing xenogeneic pig thymus Immune response Positive Immune response Negative immune response Immune tolerance Effector T cells Effector T cells Regulatory T cells Regulatory T cells Immune response Immune tolerance Graft rejection Graft survival Zhao Y. Medical Hypothesis 2022 Positive Immune responserejection Negative immune response Immune tolerance Immunisuppressive agents CsA, Rapa, antiCD4 mAb, irradiation ? Graft rejection Regulatory CD4+CD25+Foxp3+T cells Effector T cells Zhang LJ J Cell Phy 2022 Liu GW Immunology 2022 Yi HF Exp Rev of CLin Immunol 2022 A % of CD4+ CD25+ /CD4+ cells ** 0 5 10 15 20 25 30 B Control CsA Spleen LNs Total cell no of CD4+ CD25+ cells (X106) * 0 1 2 3 Control CsA Spleen LNs CsA significantly decreased the percentages of CD4+CD25+Treg cells in spleens Wang HJ, Zhao L, Sun ZY, Sun LG, and Zhao Y. Transplantation 2022. C ** * * ** * 0 25 50 75 100 125 150 3 HTdR uptake (cpm103 ) 0 1 2 4 0 5 10 15 20 25 0 50 100 150 200 3 HTdR uptake (cpm103 ) 3 HTdR uptake (cpm103 ) 0 1 2 4 0 1 2 CD4+CD25+ cell No (X103) CD4+CD25+ cell No (X103) CD4+CD25+ cell No (X103) B A * * * Control CsA Control CsA Control CsA CsA significantly inhibited the function of CD4+CD25+Treg cells in mice Wang HJ, Zhao L, Sun ZY, Sun LG, and Zhao Y. Transplantation 2022. ConA MLRC57 MLRC3H control rapa 0 5 10 15 20 25 30 % of CD4+ cells *** B C 0 5 10 15 20 25 % of CD4+ CD25high cells in CD4+cells ** 0 1 2 3 4 % of CD4+ CD25 high cells A Qu Yanyan et al, Transpl Immunol 2022 Rapamycine relatively enhanced Treg cell levels in spleens 3HTdR uptake (cpm103 ) 3HTdR uptake (cpm103 ) 0 10 20 30 40 50 60 70 80 90 0 5 10 20 40 control rapamycin CD4+CD25 CD4+CD25+ cell No (X103) 0 20 40 60 80 100 120 0 5 10 20 CD4+CD25+ cell No (X103) A B B6 CD4+CD25+ : control rapatreated Sens. B6 T cells: + + + + Balb/c SPMs: + + + + 0 40 80 120 160 200 P＞ ** ** ear thickness change (um) C 24 hours ** ** ** ** ** ** ** ** * ** ** * Qu Yanyan et al, Transplant Immunol 2022 Rapamycine did not change the function of Treg cells CsA具有抑制調(diào)節(jié)性 T細(xì)胞的功能，而Rap則對(duì)調(diào)節(jié)性 T細(xì)胞無(wú)明顯影響。 對(duì)抗 CD4 抗體、電離輻射、阿斯匹林、MMF、 LEF等對(duì)調(diào)節(jié)性 T細(xì)胞的影響進(jìn)行了研