大鼠(rat)血管內(nèi)皮生長(zhǎng)因子(vegf)-newa-在線瀏覽
2024-10-03 07:14本頁(yè)面
【正文】 determined by paring the . of the samples to the standard curve.Sample collection and storagesSerum Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000g. Remove serum and assay immediately or aliquot and store samples at 20℃ or 80℃.Avoid repeated freezethaw cyclesPlasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000g at 28℃ within 30 minutes of collection. Store samples at 20℃or 80℃. Avoid repeated freezethaw cycles.Cell culture supernates and other biological fluids Remove particulates by centrifugation and assay immediately or aliquot and store samples at 20℃or 80℃. Avoid repeated freezethaw cycles.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1. Standard microplate reader(450nm)2. Precision pipettes and Disposable pipette tips.3. 37 ℃ incubatorPrecautions1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 28176。C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard*6tubes*6tubesSample DiluentHRPConjugate reagent2
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