【正文】
oupling protein(UCP)主要存在于動(dòng)物及一些高等植物中。 HOW TO EXPRESS UCP1 IN E. COLI? ?Signal peptide ?Avoid of aggregation ?Activator: free fatty acid? ?Cofactor: CoQ? 3) MUTATE ATP SYNTHASE ?Uncouple the ATP synthesis and proton transport ?Target sites: β ,δ etc ?β S174L: decrease enzyme activity but remain the coupling to proton tr