【正文】
況下,微生物為了獲得滿(mǎn)足生長(zhǎng)需求的能量,勢(shì)必會(huì)增大對(duì)營(yíng)養(yǎng)物質(zhì)的代謝消耗。 HOW TO EXPRESS UCP1 IN E. COLI? ?Signal peptide ?Avoid of aggregation ?Activator: free fatty acid? ?Cofactor: CoQ? 3) MUTATE ATP SYNTHASE ?Uncouple the ATP synthesis and proton transport ?Target sites: β ,δ etc ?β S174L: decrease enzyme activity but remain the coupling to proton tr