【正文】 ...............................................................................4 主要藥品和試劑 ............................................................................................................................................4 實驗儀器 ........................................................................................................................................................5 培養(yǎng)基 ............................................................................................................................................................6 選擇性培養(yǎng)基 .......................................................................................................................................6 葡萄糖氧化發(fā)酵培養(yǎng)基 ......................................................................................................................6 甲基紅試驗和 VP 試驗培養(yǎng)基 .........................................................................................................7 3. 實驗方法及原理 .............................................................................................................. 7 降解丙烯酰胺菌篩選流程 ...........................................................................................................................7 菌株的富集 ...................................................................................................................................................7 菌株的分離純化 ...........................................................................................................................................8 菌種的生理生化鑒定 [45] .............................................................................................................................8 革蘭氏染色 ...........................................................................................................................................8 接觸酶試驗 ...........................................................................................................................................8 甲基紅試驗 ...........................................................................................................................................8 VP 試驗 .................................................................................................................................................8 葡萄糖氧化發(fā)酵實驗 ...........................................................................................................................9 重絡(luò)酸鉀法測 COD【 68】 ..............................................................................................................................9 丙烯酰胺降解率的測定 ...............................................................................................................................9 丙烯酰胺含量的測定 ................................................................................................................................ 10 4. 結(jié)果與分析 .................................................................................................................... 11 降解丙烯酰胺菌的分離 .............................................................................................................................11 優(yōu)勢菌的菌體染色及形態(tài)鑒定 ................................................................................................................11 II 菌體染色 .............................................................................................................................................11 生理生化鑒定 .................................................................................................................................... 12 菌株生長條件優(yōu)化 ..................................................................................................................................... 12 降解時間的影響 .........................................................................................................