分子生物學-05真核轉錄-文庫吧資料
2024-10-25 07:35本頁面
【正文】 ,tRNA processing,Yeast tRNA splicing involves cutting and rejoining,rRNA processing,樹立質(zhì)量法制觀念、提高全員質(zhì)量意識。 catalyzed by the enzyme poly(A) polymerase the poly(A)binding proteins (PABP) binds on poly(A) tail, One PABP monomer of ~70 kD is bound every 1020 bases of the poly(A) tail. Removal of the poly(A) tail precedes the degradation of certain mRNAs。 guanine7methyltransferase. cap 1: the predominant type of cap in all eukaryotes except unicellular organisms。 tRNAs and other small RNAs (5S rRNA)transcription. inhibited or not by aamanitin. All eukaryotic RNA polymerases are large proteins, 500 kD, 814 subunits.,RNA polymerases II,The largest subunit has a carboxyterminal domain (CTD), which consists of multiple repeats of a consensus sequence of 7 amino acids. The sequence is unique to RNA polymerase II. The RNA polymerase activities of mitochondria and chloroplasts are smaller, and resemble bacterial RNA polymerase.,2. Promoter,RNA polymerase I promoter has two sequence components,Core promoter: ~70 bp. UBF1: a single polypeptide, it binds to both regions (GCrich), after which SL1 can bind. RNA pol I then binds to the core promoter. UBF1: SL1: 4 subunits, one subunitTBP interacts with RNA polymerase. SL1 resembles sigma factor.,Deletion analysis shows that the promoter
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