ｂ細胞和抗體bcell-文庫吧資料
2024-10-08 20:53本頁面
【正文】 OPS KAPPA ALLELE B REARRANGES VJ, ALLELE B PRODUCTIVE NONPRODUCTIVE NAIVE IMMATURE B CELL DEVELOPS NO FURTHER IgL REARRANGEMENT LIGHT CHAIN ISOTYPE EXCLUSION LAMBDA GENE REARRANGES KAPPA GENE ETC. NEED PRODUCTIVE IgL REARRANGEMENT FOR FORMATION OF IMMATURE NAIVE B CELL IN FRAME NOT IN FRAME CONSEQUENCES OF NONPRODUCTIVE KAPPA IgL (Expresses Kappa) SIMILAR TO KAPPA IgH ?chain IgL expression BCR expression (IgM) Na239。 DJ: Site of diversification in 10 amino acid sequence V CDR1 CDR2 FR1 FR2 FR3 J C 1 FR4 V DJ D J D CDR3 Sequence variations in CDR3 affect epitope binding! IMPRECISE RECOMBINATION T C A C A T V SEGMENT D SEGMENT C A T T C A HISSER T C A SER C A T T C A A GLN C A U5192 C A T T C A N REGION DIVERSITY: BASES ADDED at JUNCTIONS N = A, T , C, or G N N N IN FRAME N N N N ADDED BY TdT (TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE) V V OUT OF FRAME D SEGMENT D SEGMENT VDJ JUNCTION DJ JUNCTION J J NOTE: DIVERSITY SEGMENT CAN BE READ IN MANY READING FRAMES! 15:1 ARE THESE NREGION ADDITIONS IN FRAME OR OUTOFFRAME? N = A, T , C, or G N N N IN FRAME N N N IN FRAME ADDED BY TdT (TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE) V V D SEGMENT D SEGMENT VDJ JUNCTION DJ JUNCTION J J U5201 V H C H REGION REGION CDR1 CDR2 CDR3 CDR1 CDR3 EPITOPE U511E SIMPLIFIED VIEW AS 6 CDRs (3 FROM IgH AND 3 FROM IgL) MAKE UP THE SITE ON THE ANTIBODY WHERE ANTIGENIC DETERMINANTS (EPITOPES) ARE BOUND CHAIN, REGIONS, DOMAINS, CDR’s, EPITOPE BINDING IgH ?chain Surrogate light chain preB cell Receptor (preBCR) LARGE preB CELL: preBCR EXPRESSION Expression of preBCR is a trigger to initation of IgL gene rearrangement: Transition to: Small preB cell SMALL preB CELL: IgL rearrangement IgL GENE ORGANIZATION separate genes for ? amp。 DOMAINS DOMAINS Protein Splicing of IgH Heavy Chain mRNA for SECRETION nascent mRNA C?1 C?1 C?1 C?4/SC M Cyto. mature mRNA for secretion AAAAAA M and Cytoplasmic exons not included in mRNA of ? chain of secreted IgM V D J C 1 IgH PROTEIN CHAIN CDR1 CDR2 CDR3 FR = FRAMEWORK REGION CDR = COMPLEMENTARITY DETERMINING REGION = HVR HVR = HYPERVARIABLE REGION REVIEW: WUKABAT PLOT for variability vs amino acid position U511D V H C H V D J C?1 C?2 C?3 C?4 DOMAINS ILLUSTRATED === Regions FR1 FR2 FR3 FR4 V REGION H JUNCTIONS BETWEEN D amp。 IgD Na23
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