【正文】
,用無菌水洗滌4~5次后,接種到含有15ml培養(yǎng)基(經(jīng)過高壓蒸汽滅菌,不加植物生長物質(zhì)的MS培養(yǎng)基)的試管中,置于溫度22~30℃,光照時間14h/d,光強(qiáng) 3000lx的條件下培養(yǎng)。和其它培養(yǎng)基的基本成分相比,MS培養(yǎng)基中硝酸鹽含量大,而且還有一定數(shù)量的銨鹽,因此培養(yǎng)基中含有大量氮、鉀,這是它的顯著特點。MS培養(yǎng)基的無機(jī)養(yǎng)分的數(shù)量和比例比較合適,足以滿足植物細(xì)胞在營養(yǎng)上和生理上的需要。關(guān)鍵詞:生長素;細(xì)胞分裂素;愈傷組織;組織培養(yǎng)Plant Growth Regulators on Brassica hypocotyl callus inductionBiotechnology 06 (2) classes Zhang XinAbstract:MSbased medium, add the plant growth regulators auxin and cytokinin, on the cabbage hypocotyl callus tissue culture for analysis of auxin and cytokinin in the process of callus tissue culture of callus Induction and growth of, for callus tissue culture plants for reference.Key words: auxin;cytokinin;callus; tissue cultureMS培養(yǎng)基是目前應(yīng)用最廣泛的一種培養(yǎng)基,是一種具有較高的無機(jī)鹽濃度,能夠滿足組織生長所需的礦質(zhì)營養(yǎng),還能加速愈傷組織和培養(yǎng)物的生長[1]。5433關(guān)鍵詞:生長素;細(xì)胞分裂素;愈傷組織;組織培養(yǎng)Different Plant Growth Regulators on Brassica hypocotyl callus inductionBiotechnology 06 (2) classes Zhang XinAbstract:MSbased medium, add the plant growth regulators auxin and cytokinin, on the cabbage hypocotyl callus tissue culture for analysis of auxin and cytokinin in the process of callus tissue culture of callus Induction and growth of, for callus tissue culture plants for reference.Key words: auxin;cytokinin; callus;tissue culture目 錄1 引言3. KT和2,4D對愈傷組織生根的影響。江蘇農(nóng)林職業(yè)技術(shù)學(xué)院 畢 業(yè) 設(shè) 計(論 文) 不同植物生長調(diào)節(jié)物質(zhì)對甘藍(lán)下胚軸愈傷組織誘導(dǎo)的影響