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【正文】 und that Eg5 can tether microtubule plusends, suggesting an additional microtubulebinding mode for Eg5. Our results demonstrate how members of the kinesin5 family are likely to function in mitosis, pushing apart interpolar microtubules as well as recruiting microtubules into bundles that are subsequently polarized by relative sliding. We anticipate our assay to be a starting point for more sophisticated in vitro models of mitotic spindles. For example, the individual and bined action of multiple mitotic motors could be tested, including minusenddirected motors opposing Eg5 motility. Furthermore, Eg5 inhibition is a majo
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