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adenas, S., Stuart, ., Harper, ., Roebuck, ., [14]Garratt, M., Vasilaki, A., Stockley, P., McArdle, F., Jacksonn, M., Hurst, ., 2011. Is oxidative stress a physiological cost of reproduction? An experimental test in house mice. Proc. R. Soc. B. 278, 1098–1106. [15] Golozoubova, V., Hohtola, E., Matthias, A., Jacobsson, A., Cannon, B., Nedergaard, J., 2001. Only UCP1 can mediate adaptive nonshivering thermogenesis in the cold. FASEB J. 15, 2048–2050. [16]孔勇,宋學(xué)琴,[J].醫(yī)學(xué)綜述,2010,第16卷第24期. [17] Gordon, ., 1993. Temperature regulation in laboratory rodents. Cambridge University Press. [18] Gordon, ., 2012. Thermal physiology of laboratory mice: Defining thermoneutrality. J. Therm. Biol. 37, 654685. 指導(dǎo)教師審核意見(jiàn): 簽 名:年 月 日6。用2μL的cDNA樣品作為模板,使用解偶聯(lián)蛋白的目的基因的特定引物進(jìn)行PCR反應(yīng)。熒光定量PCR分析使用Trizol試劑(TAKARA,中國(guó)大連)提取褐色脂肪組織、肝、心臟、骨