【正文】 e authors are deeply grateful to Dr Pawan Kapur, Director,Dr P. K. Jain and Mr P. K. Goel, Scientists of CSIO,Chandigarh, and Dr Jasbinder Kaur, Acting Head, Department of Biochemistry, GMCH32, Chandigarh, for providing the necessary facilities and help during the progress of work and clinical evaluation of the system. The project was sponsored by the Department of Science and Technology, New Delhi, India. REFERENCES [1] G. G. Guilbault, Study of Handbook of Enzymatic Methods of Analysis, Marcel Dekker, New York, NY, USA, 1976. [2] R. Haeckel, “General principles for the classification of analysers,”Journal of Automated Methods and Management in Chemistry, vol. 10, no. 4, pp. 164–166, 1988. [3] D. C. Harris, Quantitative Chemical Analysis, W. H. Freeman amp。C, and 37176。 a light source, an optical module, a filter wheel, a quartz cuvette with reaction mixture, a photo detector, and signal processing circuitry based on microcontroller. Overall systemdesign is shown in Figure 4. The block diagram can be subdivided into the following major parts: (i) the microcontroller and memories, (ii) peripherals and interfaces. System design is based around 80C31 microcontroller [4] connected through address bus, data bus, and control bus to the 64 kbytes of EPROM 27C512 for monitor and control program, 24 kbytes of RAM with battery backup for temporary data storage, 24hour results storage capacity, and peripheral I/O devices 8255 s are used for interfacing 32key keyboard, 12bit A/D converter, 40 column thermal mini printer, and 30 characters 8 lines alphanumeric/graphic LCD display. Alphanumeric keyboard contains various function keys, numeric keys and aspirate and RESET key for selecting various functions of the system and parameter LCD is used for displaying date and time by realtime clock, various menus, parameters and data entered from keyboard as well as patient’s results and kiic graphs as required in some test parameters. 12bit A/D converter converts analog signal from photo detector and preamplifier into digital form. 40 column thermal mini printer is used for hard copy of the parameters stored, patient test results, collection report, and kiic graphs as displayed on the LCD. These graphs facilitate authenticity of the test results. (iii) Peltierbased temperature controller System developed is used to determine both enzyme activity and substrate concentration in biological fluids at different temperatures 25 176。 10 中文 3340 字 畢業(yè)論文（設(shè)計） 外文翻譯 譯文題目 : 學(xué)生姓名： 學(xué) 號： 專 業(yè)： 生物醫(yī)學(xué)工程 方 向： 醫(yī)療器械 指導(dǎo)教師： 11 Design and Development of MicrocontrollerBased Clinical Chemistry Analyser for Measurement of Various Blood Biochemistry Parameters S. R. Taneja, R. C. Gupta, Jagdish Kumar, K. K. Thariyan, and Sanjeev Verma, Journal of Automated Methods amp。 Management in Chemistry, India Clinical chemistry analyser is a highperformance microcontrollerbased photometric biochemical analyser to measure various blood biochemical parameters such as blood glucose, urea, protein, bilirubin, and so forth, and also to measure and observe enzyme growth occurred while performing the other biochemical tests such as ALT (alkaline amino transferase), amylase, AST (aspartate amino transferase), and so forth. These tests are of great significance in biochemistry and used for diagnostic purposes and classifying various disorders and diseases such as diabetes, liver malfunctioning, renal diseases, and so forth. An inexpensive clinical chemistry analyser developed by the authors is described in this paper. This is an open system in which any reagent kit available in the market can be used. The system is based on the principle of absorbance transmittance photometry. System design is based around 80C31 microcontroller with RAM, EPROM, and peripheral interface devices. The developed system incorporates light source, an optical module, interference filters of various wave lengths, peltier device for maintaining required temperature of the mixture in flow cell, peristaltic pump for sample aspiration, graphic LCD display for displaying blood parameters, patients test results and kiic test graph, 40 columns mini thermal printer, and also 32key keyboard for executing various functions. The lab tests conducted on the instrument include versatility of the analyzer, flexibility of the software, and treatment of sample. The prototype was tested and evaluated over 1000 blood samples successfully for seventeen blood parameters. Evaluation was carried out at Government Medical College and Hospital, the Department of Biochemistry. The test results were found to be parable with other standard instruments. 1. I