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【正文】 nd Astragalus membuanaceus by high performance liquid chromatography–mass spectrometry. Chin J Anal Chem 33:1676–1680 9. Qi LW, Yu QT, Yi L, Ren MT, Wen XD, Wang YX, Li P (2021) Simultaneous determination of 15 marker constituents in various Radix astragli preparations by solid phase extraction and high performance liquid chromatography. J Sep Sci 3 1:97–106 10. Huang X, Liu Y, Song FR, Liu ZQ, Liu SY (2021) Studies on principal ponents and antioxidant activity of different Radix astragali samples using highperformance liquid chromatography/ electrospray ionization multiplestage tandem mass spectrometry. Talanta 78:1090–1101 11. Zu YG, Yan MM, Fu YJ, Liu W, Zhang L, Gu CB, Efferth T (2021) Determination and quantification of astragalosides in Radix astragali and its medicinal products using LC–MS. J Sep Sci 32:517–525 12. Rios JL, Waterman PG (1997) A review of the pharmacology and toxicology of Astragalus. Phytother Res 11:411–418 13. Hirotani M, Zhou Y, Lui H, Furuya T (1994) Cycloartane triterpene glycosides from the hairy root cultures of Astragalus membranaceus. Phytochemistry 37:1403–1407 14. Subarnas A, Oshima Y, Hikino H (1991) Isoflavans and a pterocarpan from Astragalus mongholicus. Phytochemistry 30:2777–2780 15. Zhu HY, Zhang YY, Ye G, Li ZX, Zhou P, Huang CG (2021) In vivo and in vitro antiviral activities of calycosin7Of3Dglucopyranoside against Coxsackie virus B3. Biol Pharm Bull 32:68–73 16. Huang CG, Zhang YY, Zhu HY, Zhang ZQ, Wang XL (2021) Astragaloside IV used for the treatment of viral myocarditis. Patent CN1502336 。 另外,生物測(cè)定和 HPLC–DAD–ESI–MS/MS分析的聯(lián)合應(yīng)用是一種快速鑒定植物藥生物活性成分的有效途徑 。 在已鑒定的化合物中 毛蕊異黃酮 7Oβ D吡喃葡萄糖苷 和 黃芪甲甙 IV曾報(bào)道有生物學(xué)活性。 討論: 盡管質(zhì)量控制往往成為阻礙中藥發(fā)展的障礙, 近來(lái)也依然提出了一些方法并且 取得了一些成績(jī)。 Fig. 2 HPLC–DAD–ESI–MS分析 70%F. a HPLC–UV 譜 , 檢測(cè)在 280nm處。化合物 19在 933處有 [M + Na]+離子,它的 MS/MS譜顯示在 753處有 [M+NaGluH2O]+重要離子,通過(guò)查看黃芪中的已知皂苷,只有乙酰黃芪苷 I由以上數(shù) 據(jù)組成。通過(guò)查看黃芪中的已知皂苷,黃芪皂苷 I和異黃芪皂苷 I的數(shù)據(jù)和以上相符。它們?cè)?891處有 [M + Na]+離子,它們的 MS/MS譜在 711處有一個(gè)重要的離子 [M
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