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三種測(cè)序dna模板制備方法的比較(參考版)

2024-09-02 05:53本頁(yè)面
  

【正文】 實(shí)驗(yàn)所用到的 3 種方法均適用于基因組 DNA 的測(cè)序, 而 96 管集合板法更適用于甲基化 DNA 的測(cè)序。利益沖突:課題未涉及任何廠家及相關(guān)雇主或其他經(jīng)濟(jì) 組織直接或間接的經(jīng)濟(jì)或利益的贊助。7(4):568571.[21][2][22][3][23][4][24][5][25][6][26][7][8][9][10][11][12][13][14][15][16]2822萬(wàn)方數(shù)據(jù). Box 1200, Shenyang110004 來(lái)自本文課題的更多信息作者貢獻(xiàn):實(shí)驗(yàn)設(shè)計(jì)為通訊作者,實(shí)驗(yàn)實(shí)施為第一、二 三作者,評(píng)估為所有作者,均經(jīng)過(guò)系統(tǒng)培訓(xùn)。9:387402.Xiao L, Zhang J, Sirois P, et al. A new strategy for next generation sequencing: merging the Sanger39。33(8):829846. 楊旭,焦睿,楊琳, 略[J].遺傳, 2010,33(8):829846.Pareek CS, Smoczynski R, Tretyn A. Sequencing technologies and genome sequencing. J Appl Genet. 2011。7:149158.Casci T. DNA sequencing: Exome sequencing technologiespared. Nat Rev Genet. 2011。20(2):128134.Juan C, Armando A. Determination of the in vivo structural DNAloop organization in the genomic region of the rat albumin locus by means of a topological approach. DNA Res. 2010。12(5):607610.Whetten RW, Sof237。183(2):210216.Yang Y, Hebron HR, Hang J. A method for preparing DNAsequencing templates using a DNAbinding microplate. J BiomolTech. 2009。39(12):e81.Laura T, Michael W, and Linda J, et al. Differences in replication of a DNA template containing an ethyl phosphotriester by T4 DNA polymerase and Escherichia coli DNA polymerase I. Nucleic AcidsRes. 2003。6(11):e26054.Quail MA, Matthews L, Sims S, et al. Genomic libraries: II. subcloning, sequencing, and assembling largeinsert genomic DNA clones. Methods Mol Biol. 2011。[18][19]4參考文獻(xiàn)[20][1]Wang H, Wang XJ, Zhen YS, et al. Zhongguo FenziXinzangbingxue Zazhi. 2004。1:4446. 宋艷斌,馬文麗,[J].生物技 術(shù)通報(bào),2005,1:4446.序失敗。55(4):641658.Yu Y, Haroun RH, Jun H, et al. A Method for Preparing DNA Sequencing Templates Using a DNABinding Microplate. J BiomolTech. 2009。43(4):551563.Kato K. Impact of the next generation DNA sequencers. Int J ClinExp Med. 2009。rzer I, Guelly C, Trajanoski S, et al. The impact ofPCRgenerated rebination on diversity estimation of mixedviral populations by deep sequencing. J Virol Methods. 2010。22(2):5359.Pardo C
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