【正文】 4: Sheil et al. 2022 Multireplicon genome architecture Claesson, Li et al. PNAS, 2022, 103: 67186723 (cover article) ? NAcetylneuraminate ? Sorbitol PTS and related ? Pyruvate related metabolism ? Gluconeogenesis ? Rhamnose metabolism ? Ribose metabolism ? Mannose PTS ? Replication proteins pMP118 242,436 bps 50000 100000 150000 202200 Metabolic capabilities encoded by pMP118 ? Bacteriocin ABP118 Genomics reveals new metabolic capability Red: pMP118 genes Green: chromosomal genes Amber: encoded by both Claesson et al. PNAS, 2022, 103: 67186723 0 CONTROL 1 GLYCEROL 2 ERYTHRITOL 3 DARABINOSE 4 LARABINOSE UCC118 6 DXYLOSE 7 LXYLOSE 8 ADONITOL 9 ?METHYLDXYLOSIDE 10 GALACTOSE 11 GLUCOSE 12 FRUCTOSE 5 DRIBOSE 13 MANNOSE 14 SORBOSE 15 RHAMNOSE 16 DULCITOL 17 INOSITOL 18 MANNITOL 19 SORBITOL 20 ?–METHYLDMANNOSIDE 21 ?–METHYLDGLUCOSIDE 22 NACETYLGLUCOSAMINE 23 AMYGDALIN 24 ARBUTIN 25 ESCULIN 26 SALICIN 27 CELLOBIOSE 28 MALTOSE 29 LACTOSE 30 MELIBIOSE 31 SACCHAROSE 32 TREHALOSE 33 INULIN 34 MELEZITOSE 35 RAFFINOSE 36 STARCH 37 GLYCOGEN 38 XYLITOL 39 GENTIOBIOSE 40 DTURANOSE 41 DLYXOSE 42 DTAGATOSE 43 DFUCOSE 44 LFUCOSE 45 DARABITOL 46 LARABITOL 47 GLUCONATE 48 2KETOGLUCONATE 49 5KETOGLUCONATE AH4231 AH4331 AH43310 AH43324 DSM20492 DSM20555 NCIMB8816 NCIMB8817 NCIMB8818 CCUG27530B CCUG38008 CCUG43299 CCUG45735 CCUG47825 L. salivarius subsp. salicinius DSM20554 L. salivarius subsp. salivarius NCIMB702343 CCUG44481 CCUG47171 CCUG47826 JCM1040 JCM1042 JCM1044 JCM1045 JCM1046 JCM1047 JCM1230 L. salivarius (subspecies unidentified) UCC119 01M14315 LMG14476 LMG14477 L21 Rhamnose Salicin Li et al. IJSEM, 2022 56: 23972403. Ribose Existing subspecies not supported groEL phylogeny L. salivarius UCC118 CCUG27530B AH43324 NCIMB8818 AH43310 JCM1230 CCUG47826 CCUG47171 LMG14477 NCIMB702343 CCUG38008 NCIMB8817 LMG14476 JCM1045 DSM20555 JCM1046 CCUG45735 AH4231 AH4331 DSM20492 DSM20554 14C7 CCUG44481 CCUG47825 01M14315 CCUG43299 JCM1040 L21 NCIMB8816 JCM1042 JCM1044 100 60 35 66 91 61 98 97 83 80 98 89 82 81 96 99 95 65 Subspecies salivarius Subspecies salicinius Emended species description A single species Lactobacillus salivarius Li et al. IJSEM, 2022 56: 23972403. L. salivarius megaplasmid repertoire ? 10 strains hybridized to LSL_1739 (repA), LSL_1740 (repE) 800 1600 2400 3200 4000 4800 5600 RepA RepE ParA LSL1739 LSL1740 LSL1741 Claesson, Li et al. PNAS, 2022, 103: 67186723 33 L. salivarius strains collected ? Cork human GIT isolates: UCC118 (433118), 4331, 4231, 43310, 43324, 43348, 43361 ? Other human GIT isolates: JCM1040, JCM1042, JCM1044, JCM 1045, L21 ? Human saliva: DSM20492, DSM20554, DSM20555, NCIMB8816 ? Human clinical isolates: CCUG27530B, CCUG38008, CCUG43299, CCUG45735, CCUG47825, CCUG47171, CCUG47826, 01M14315 ? Animal GIT (faeces): UCC119, JCM1046, JCM1047, JCM1230, NCIMB8817 ? Animals: CCUG44481, LMG14476, LMG14477 ? Food (cheese): NCIMB8818 ? Unknown origin: NCIMB702343 9 7 k b1 4 5 . 5 k b1 9 4 k b2 4 2 . 5 k b2 9 1 k b1 M b4 8 . 5 k bAH4 3 3 1 0U C C1 1 801M1 4 3 1 5J C M1 0 4 0S1 + + + + + + + + + + +J C M1 0 4 2J C M1 0 4 5J C M1 0 4 6J C M1 0 4 7AH4 3 3 4 8L2 1 N C I M B8 8 1 8LM G1 4 4 7 7LM G1 4 4 7 6C C U G2 7 5 3 0 BC C U G3 8 0 0 8S1 + + + + + + + + + +C C U G4 3 2 9 9C C U G4 5 7 3 5C C U G4 7 8 2 5C C U G4 4 4 8 1C C U G4 7 1 7 1C C U G4 7 8 2 6LM G1 4 4 7 7LM G1 4 4 7 6C C U G2 7 5 3 0 BC C U G3 8 0 0 8+ + + + + + + + + +C C U G4 3 2 9 9C C U G4 5 7 3 5C C U G4 7 8 2 5C C U G4 4 4 8 1C C U G4 7 1 7 1C C U G4 7 8 2 69 7 k b1 4 5 . 5 k b1 9 4 k b2 4 2 . 5 k b2 9 1 k b1 M b4 8 . 5 k bAH4 3 3 1 0U C C1 1 801M1 4 3 1 5J C M1 0 4 0+ + + + + + + + + + +J C M1 0 4 2J C M1 0 4 5J C M1 0 4 6J C M1 0 4 7AH4 3 3 4 8L2 1 N C I M B8 8 1 8All tested L. salivarius strains (n=33) hybridized to LSL_1739 (repA), LSL_1740 (repE), LSL_1741 (parA) Li et al. J. Bacteriol. 2022, 189: 61286139. Statistics of L. salivarius repAtype MPs DSM20555 LMG14476 LMG14477 DSM20554 UCC118 AH43310 AH43324 DSM20492 CCUG44481 JCM1047 CCUG47171 JCM1046 JCM1045 CCUG47825 CCUG45735 CCUG27530B CCUG47826 CCUG43299 CCUG38008 AH4231 AH4331 01M14315 UCC119 AH43348 NCIMB8818 JCM1040 L21 NCIMB8816 JCM1042 JCM1044 NCIMB702343 NCIMB8817 380 kb 290 kb 270 kb 260 kb 242 kb 240 kb 240 kb 240 kb 240 kb 240 kb 240 kb 230 kb 220 kb 220 kb 220 kb 220 kb 220 kb 218 kb 215 kb 210 kb 210 kb 200 kb 195 kb 195 kb 195 kb 195 kb 190 kb 180 kb 180 kb 180 kb 160 kb 145 kb JCM1230 100 kb 218 ? 47 kb (n=33) 300 kb: 1 200300 kb: 22 100200 kb: 10 Li et al. J. Bacteriol. 2022, 189: 61286139