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參考文獻(xiàn):[1] Faerber C.Future demands on cotton fiber quality in the textile industry[R]. Beltwide cotton conferences, 1995.[2] . 棉花產(chǎn)量與品質(zhì)改進(jìn)新對策[J]. 棉花學(xué)報,2001,13(1):54~58. [3] Wendel J F,Albert V A . Phylogenetics of the cotton genus (Gossypium): Characterstate weighted parsimony analysis of chloroplastDNA restriction site data and its systemic and biogeographic implication[J]. Syst. Bot, 1992, 17:115~143.[4] 項時康, 余楠, 胡育昌, 等. 論我國棉花質(zhì)量現(xiàn)狀[J]. 棉花學(xué)報,1999, 11(1):1~10.[5] 黃滋康.中國棉花品種及其系譜[M]. 北京: 中國農(nóng)業(yè)出版社, 1996.[6] 潘家駒. 棉花育種學(xué)[M]. 北京:中國農(nóng)業(yè)出版社,1998.[7] 梁正蘭, 姜茹琴, 鐘文南, 等. 棉花種間雜交技術(shù)創(chuàng)新及育種程序的建立[J]. 中國科學(xué)C緝, 2001, 1(31):120~124. A[8] 朱乾浩, 季道藩. 棉花輻射誘變育種研究進(jìn)展[J]. 棉花學(xué)報, 1997, 9(3):113~119.[9] 何卓培, 周慶棋, 徐淑平, 等. 輻射誘變育成棉花高衣分品系[J]. 實驗生物學(xué)報, 2001, 34(4):51~54.[10] 張?zhí)煺妫?靖深蓉. 棉花雄性不育雜交種選育的理論與實踐[M]. 北京:中國農(nóng)業(yè)出版社, 1997.[11] 李悅有, 尹學(xué)德. 細(xì)胞質(zhì)雄性不育彩色棉雜種優(yōu)勢的表現(xiàn)[J]. 浙江大學(xué)學(xué)報(農(nóng)業(yè)與生命科學(xué)版), 2002,28(1):7~10. [12] Culp T W, Harrel D C, Kerr T. Some genetic implications in the transfer of high fiber strength genes to upland[J]. Crop Sci, 1979, 19:481~484.[13] Culp T W.Breeding methods for improving yield and fiber quality of upland cotton[J]. Crop Sci,1973, 13:686~689.[14] Du C F, Li P B, Li R Z. Research advances in molecular basis of the variations and QTLs cloning of quantitative plant traits[J]. Acta Botanica Sinica, 2005, 25(12):2575~2580.[15] 方宣鈞, 吳為人, 唐紀(jì)良. 作物DNA標(biāo)記輔助育種[M]. 北京: 科學(xué)出版社, 2001.[16] Reinisch A J,Dong J M, Brubaker C L, et al. A detailed RFLP map of cotton Gossypium hirsutumGossypium barbadense chromosome organization and evolution in a disomic polyploid genome [J]. Genetics, 1994, 138(3):829~847.[17] Shappley Z W, Jenkins J K, Watson C E,et al. Establishment of molecular markers and linkage groups in tow F2 population of upland cotton[J]. Theor Appl Genet, 1996,92: 915~919.[18] Ullao M, Meredith W R. Genetic linkage map and QTL analysis agronomic and fiber quality traits in an intraspecific population[J]. Journal of Cotton Science, 2000, 4: 161~170. [19] Ullao M, Meredith W R, Shappley Z W, et al. RFLP genetic linkage maps from F2:3 populations and a join map of Gossypium hirsutum[J]. Theor Appl Genet, 2002, 104:200~208. [20] Kohel R J, Yu J, Park Y H, et al. Molecular mapping and characterization of traits controlling fiber quality in cotton[J]. Euphytica, 2001, 121:163~172. [21] Ullao M, Meredith W R. Genetic linkage map and QTL analysis agronomic and fiber quality traits in an intraspecific population[J]. Journal of Cotton Science, 2000, 4:161~170.[22] Jiang C X, Wright R J, EtZik K M, et al. Polyploid formation created unique avenues for response to selection in Gossypium (cotton) [J]. Pro Natl Acad Sci USA, 1998, 95: 4419~4424.[23] Lacape J M,Nguyen T B, Thibivilliers S, et al. A bined RFLPSSRAFLP map of tetraploid cotton based on a Gossypium hirsutumGossypium barbadense backcross population[J]. Genome,2003,46:612~626.[24] Nguyen T B, Giband M, Brottier P, et al. Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers [J]. Theor Appl Genet, 2004, 109:167~175.[25] Han Z G, Wang C B, Song X L, et al. Characteristics development and mapping of Gossypium hirsutum derived ESTSSRs in allotetraploid cotton[J]. Theor Appl Genet,2006,112:430~439.[26] Guo W Z, Cai C P, Wang C B, et al. A preliminary analysis of genome structure and position in Gossypium hirsutum[J]. BMC Genomics, 2008, 9:314~325.[27] Shen X L, Guo W Z,Zhu X F, et al. Molecular mapping of QTLs for fiber qualities in three diverse lines in Upland cotton using SSR markers[J]. Mol Breed,2005,15:169~181.[28] Reddy O U K,Pepper A E,Abdurakimonov I,et al. New dinucleotion and trinucleotide microsatelite maker resourse for cotton genome research[J]. The journal of cotton science,2001, 5:103~113.[29] 倪會娟, 王威, 張建, 等. 利用F2及其衍生群體定位陸地棉產(chǎn)量和纖維品質(zhì)性狀QTLs[J]. 西南大學(xué)學(xué)報(自然科學(xué)版), 2011, 33(6): 7~14.[30] 王娟, 郭旺珍, 張?zhí)煺? 渝棉1號優(yōu)質(zhì)纖維QTL的標(biāo)記與定位[J]. 作物學(xué)報, 2007, 33(12):1915~1921.[31] Rong J K, Abbey C, Bowers J E, et al. A 3347locus genetic rebination map of sequencetagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium)[J]. Genetics, 2004, 166:389~417.。引起標(biāo)記分布不均的原因是多方面的:第一,研究者篩選到的標(biāo)記可能多數(shù)是由基因組中某一亞組開發(fā)得到的;第二,遺傳連鎖圖譜中標(biāo)記數(shù)量較少也會造成染色體某些區(qū)段標(biāo)記稀疏;第三親本間在染色體某些區(qū)段缺乏多態(tài)性。D亞組7條染色體上分布有86個標(biāo)記位點。本研究所構(gòu)建遺傳連鎖圖譜A亞組標(biāo)記多于D亞組,同樣也具有標(biāo)記分布不均的現(xiàn)象。最為詳盡的遺傳連鎖圖譜是由Rong等[31]利用STS標(biāo)記構(gòu)建的,該圖譜包含2584個位點,26個連鎖群或染色體。構(gòu)建遺傳連鎖圖譜時,研究者都期望標(biāo)記位點能均勻分布于基因組上。但是陸地棉種內(nèi)多態(tài)性較低,與海島棉和陸地棉雜交群體所構(gòu)建的遺傳連鎖圖譜相比,利用陸地棉所構(gòu)建的遺傳連鎖圖譜缺點很多。倪會娟等[29]利用陸地棉品種中棉所35和渝棉1號,從8165對SSR引物中篩選出335對多態(tài)性引物,%。與上述方法相比,陸地棉種內(nèi)雜交育種也有重要地位,近年來在遺傳連鎖圖譜的構(gòu)建的過程中,有學(xué)者利用陸地棉種內(nèi)雜交群體進(jìn)行。表3檢測到的纖維品質(zhì)性狀QTLTable 3 The screened QTLs of fiber quality性狀TraitsQTL染色體Chr./LG標(biāo)記區(qū)間Interval marker位置PositionLOD貢獻(xiàn)率R2 (%)纖維長度FLFL11NAU2083bNAU2083a整齊度FUFU11NAU1071aNAU1071bFU12DOW027bDOW027c馬克隆值FMFM11NAU1369eNAU1369cFM12NAU1369cNAU3287FM13NAU3287NAU1369bFM14DPL0068cDPL0068aFM15DPL0112NAU5247aFM16NAU5461dNAU3621e比強(qiáng)度FSFS11NAU2395NAU1092aFS12NAU3621eNAU3621bFS13NAU5046dDOW027a伸長率FEFE11STV191NAU3942b3討論親本選擇是關(guān)于遺傳連鎖圖譜構(gòu)建的難易程度及圖譜的適用范圍的主