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蛋白互作研究方法ppt課件(參考版)

2025-05-05 12:03本頁面
  

【正文】 Screening Kits 誘餌蛋白載體構(gòu)建 pGADT7Rec Vector Information 文庫構(gòu)建 Constructing AD fusion libraries by rebinationmediated cloning in yeast. cDNA library of oilseed rape line RS1 constructed in yeast Yeast cells that are mating. A zygote typically has threelobed shape, the lobes representing the two haploid(parental) cells and the budding diploid cells. Library screening by mating Positive clones confirmed on SD/Ade/His/Leu/Trp/XaGal 檢測特定兩個蛋白是否互作 pGADT7 Vector Information 膜蛋白互作研究方法 ---基于泛素的酵母雙雜交體系 (matingbased Split ubiquitin system mbSUS“) 1) GAL4體系的缺點 2) MbSUS 體系 mbSUS 原理 In vivo cloning into mbSUS vec
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