sars病原與檢測(cè)ppt課件(參考版)
2025-01-08 03:30本頁面
【正文】 (檢測(cè) SARS病毒結(jié)果為陰性并不就表示病人沒有感染 SARS病毒 ) Laboratory case definition of SARS A person with symptoms and signs that are clinically suggestive of SARS AND with positive laboratory findings for SARSCoV based on one or more of the following diagnostic criteria: a) PCR positive for SARSCoV PCR positive using a validated method from: ?At least two different clinical specimens (eg nasopharyngeal and stool) OR ?The same clinical specimen collected on two or more occasions during the course of the illness (eg sequential nasopharyngeal aspirates) OR ?Two different assays or repeat PCR using a new RNA extract from the original clinical sample on each occasion of testing. b) Seroconversion by ELISA or IFA ?Negative antibody test on acute serum followed by positive antibody test on convalescent phase serum tested in parallel OR ?Fourfold or greater rise in antibody titre between acute and convalescent phase sera tested in parallel. c) Virus isolation ?Isolation in cell culture of SARSCoV from any specimen AND PCR confirmation using a validated method. Testing should only be undertaken in a national or regional reference laboratory as per WHO remendations (Use of laboratory methods for SARS diagnosis ). WHO will assist resource poor countries to confirm their first cases of SARS through laboratory collaboration. Alert, verification and public health management of SARS in the postoutbreak period WHO Remendations on interpretation of laboratory results Positive SARS diagnostic test findings a) Confirmed positive PCR for SARS virus: at least 2 different clinical specimens (eg nasopharyngeal and stool) OR the same clinical specimen collected on 2 or more days during the course of the illness (eg 2 or more nasopharyngeal aspirates) OR 2 different assays or repeat PCR using the original clinical sample on each occasion of testing b) Seroconversion by ELISA or IFA: negative antibody test on acute serum followed by positive antibody test on convalescent serum OR fourfold or greater rise in antibody titre between acute and convalescent phase sera tested in parallel c) Virus isolation: Isolation in cell culture of SARSCoV from any specimen。病毒及其遺傳物質(zhì)僅存在一段較短的時(shí)期（依賴于不同標(biāo)本種類）。目前的方法需要提高靈敏度。
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