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2)there is no need for direct staining。 ? 血清學(xué)診斷適用于深部真菌感染。 used for viruses difficult to culture Disadvantages:Potential crossreaction。used for viruses difficult to culture Disadvantages:Falsepositives and –negatives。isolate available for characterization Disadvantages:Cell culture facilities needed。 ? 新的早期診斷技術(shù):核酸雜交技術(shù)、PCR技術(shù)。 18 檢測(cè)程序 19 Diagnosis of bacterial infections ? The identification and differentiation of bacteria principally relies: ? on microbial morphology and growth variables on culturing the anisms on nonselective and selective media. Culture remains the standard for diagnosis of bacterial infection because it is the simplest,the most reliable and the most cost efficient method. Another advantage is the fact that parallel testing of the sensitivity towards antibiotics is possible. 20 ? n molecularbiological technics that provide rapid identification of infectious agents,both from culture isolates and directly within clinical specimens. ? on the detection of the antibody response to the path