【正文】
每次測量任務(wù)完成后用去離子水清洗檢測面及周圍以避免樣品殘留。 Baseline: if selected, the absorbance value of the baseline is subtracted from the absorbance of Abs1 and Abs2. 如果選擇此項(xiàng),則基準(zhǔn)線處吸光值將從 Abs1 和 Abs2中去除。因?yàn)椴恍枰獦?biāo)準(zhǔn)曲線,所以檢測快速簡便。 原文: It is remended that measuring the blanking buffer as if it were a sample: 1. Establish a blank as above “4”. 2. Measure the blanking buffer as if it were a sample as above “5”. 3. The result should be no more than at 10 mm absorbance equivalent, if not, repeat the 1 and 2. 使用圖示 SAVING THE DATA AND INFORMATION Note: Q5000 program does not save the data automatically. All data and information for the sample being measured will be lost if it is not saved. User can save the data using the “Report” function to export the data from the data table to an Excel spreadsheet. Example1: NUCLEIC ACIDS Sample Volume There is no specific requirement for the sample volume。 ?若出現(xiàn)對話框有“放棄”、“重試”、“忽略”三個選項(xiàng)時,請選擇選項(xiàng)“忽略”,使安裝繼續(xù)進(jìn)行。 二 .Q5000使用詳解 軟件安裝 插入光盤,雙擊“ Setup”文件,按照提示完成軟件安裝。 ,正確液柱如下圖所示。若擔(dān)心DNA可能因前述動作而斷裂,可改以 55℃ 加熱約一分鐘,使樣品 在檢測前呈均勻狀態(tài),以確保用于上樣檢測的 2ul樣品具有代表性。( Q5000) 工作原理 應(yīng)用液體的表面張力特性,只需要 液體樣品,便能在上下檢測臂之間拉出一個標(biāo)準(zhǔn)的小液柱,儀器檢測通過小液柱的光線的變化情況,得到溶液的吸光值,進(jìn)而計(jì)算出所含溶質(zhì)濃度。 Q5000使用高能量氙燈提供 200850nm的全光譜檢測,不需要暖機(jī),開機(jī)即可使用。搭配高感度的 CCD array檢測器,檢測吸收值可高達(dá) 100Abs (dsDNA濃度 1ng/ul5000ng/ul)大部分純化后的生物大分子幾乎都不需要稀釋濃縮即可上機(jī)檢測。 產(chǎn)品特點(diǎn) ?樣品體積?。?; ?檢測濃度范圍寬:無需濃縮或稀釋樣品就可以檢測; ?操作簡單:無需比色杯,減少了反復(fù)清洗比色杯帶來的麻煩,減少了實(shí)驗(yàn)誤差; ?檢測方便快捷:不需要預(yù)熱,開機(jī)就可使用; ?數(shù)據(jù)處理簡便:操作界面便捷,數(shù)據(jù)可輕松轉(zhuǎn)存到 Excel中。 。如若未出現(xiàn)正確液柱,應(yīng)當(dāng)擦掉液滴,重新加樣。 使用 USB數(shù)據(jù)線將儀器與電腦相連,驅(qū)動將自動安裝。 ?點(diǎn)擊主界面下的 。 however for the best accuracy and reproducibility we remend . SW and Abs (10mm): userselected wavelength and corresponding absorbance that are not utilized in any calculations. Sample Type – EC: dsDNA, ssDNA and RNA. Example2: MICROARRAY The Microarray module analyzes fluorescentlylabeled nucleic acid probes. It simultaneously measures the concentration of the fluorescent