重組人b淋巴細胞刺激因子工程菌高密度發(fā)酵-資料下載頁
2025-06-29 05:01本頁面
【正文】 批補料發(fā)酵，每升培養(yǎng)液最高可得到50 g干菌種，A600 nm可達125左右。在我們實驗中，嚴格控制補料流加的速度，在有效提高菌體產(chǎn)量的同時提高目的蛋白表達量。高密度發(fā)酵技術(shù)能夠減少培養(yǎng)體積,縮短生長周期,減少設(shè)備投資并強化下游分離提取,為工業(yè)化生產(chǎn)降低成本。本文對重組DH5a/pKKHBLyS的高密度、高表達發(fā)酵研究將可能對其它類型重組蛋白的發(fā)酵生產(chǎn)具有一定的借鑒作用。References: [1] Moore P A, Belvedere O, Orr A, et al. BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator[J]. Science, 1999, 285(5425): 260263.[2] Huntington N D, Tomioka R, Clavarino C, et al. A BAFF antagonist suppresses experimental autoimmune encephalomyelitis by targeting cellmediated and humoral immune responses[J]. Int Immunol, 2006, 18(10): 14731485.[3] Zhang J, Roschke V, Baker K P, et al. Cutting edge: a role for B lymphocyte stimulator in systemic lupus erythematosus[J]. J Immunol, 2001, 166(1): 610.[4] Cheema G S, Roschke V, Hilbert D M, et al. Elevated serum B lymphocyte stimulator levels in patients with systemic immunebased rheumatic diseases[J]. Arthritis Rheum, 2001, 44 (6):13131319.[5] Xue X, Feng G, Li M, et al. Amelioration of experimental autoimmune encephalomyelitis by BLyS autovaccine[J]. Vaccine, 2008, 26(23): 28732881.[6] 李民,樸勤,陳常慶,等. 利用恒溶氧補料分批技術(shù)高密度培養(yǎng)大腸桿菌生產(chǎn)重組人骨形成蛋白2A[J]. Chinese Journal of Biotechnology (生物工程學報),1998 , 14 (3): 270276.[7] 劉文波，柴同杰. 大腸桿菌高密度發(fā)酵研究[J]. 動物科學與動物醫(yī)學, 2001, 18(1):2729.[8] MacDonald H L, Neway J O. Effects of medium quality on the expression of human interleukin2 at high cell density in fefmentor cultures of Escherichia coli K12[J]. Appl Environ Microbiol, 1990, 56 (3): 640645.6 / 6
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