topoisomerasetypeiistructures-資料下載頁
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【導讀】pactgenome.DNA—transcription,replication,rebination,andchromosome. separation.cleavage;thatis,cutting.cutboth(2)strandsofDNA.What‘sOurType?TypeIIsomerase. TypeIIIsomerase. What‘sOurType?TypeIIsomerase. TypeIIIsomerase. Mechanism:Atwo-. throughtheopening,thebreak.topoisomerase!Summary. Conclusions. Impact. Limitations. Summary. Conclusions. Impact. Limitations
【正文】 II, we can: – Examine plex DNA and see if its geometry is changed by experimental drugs – Test inhibitors for Topoisomerase Type II to prevent DNA bending and kill the cell – Prevent rigidity in Topoisomerase Type II treatments due to builtup resistance Further Research Future Research ? Multicellular Confirmation – Is Berger and Dong‘s model conserved when the experiment is repeated on multicellular anisms ? Enzyme Shift – Following Berger and Dong‘s model bending DNA, does the enzyme undergo another conformational change prior to cutting the DNA Future Research ? Chemical basis of DNA cleavage ? Benefits of unidirectional transport – not understood ? Visualization of DNA cleavage References ―Structure and mechanism of DNA topoisomerase II‖ + James Berger amp。 Ken Dong。 Nature, Vol. 379, Jan. 1996, pp. 225 – 232 ―Simplification of DNA Topology Below Equilibrium Values by Type II Topoisomerases‖ + Valentin Rybenkov et al。 Science。 Vol. 277。 Aug. 1997。 pp. 690 – 693 ―Mechanism of topology simplification by type II DNA topoisomers‖ + Alexander Vologodskii et al。 PNAS。 Vol. 98 No. 6。 Mar. 2020。 pp. 3045 – 3049 ―An Antitumor DrugInduced Topoisomerase Cleavage Complex Blocks a Baccteriophage T4 Replication Fork in Vivo‖ + Gee Hong amp。 Kenh Kreuzer。 Molecular and Cellular Biology。 Vol 20 No. 2。 Jan. 2020。 ―Involvement of DNA topoisomerase II in the selective resistance of a mammalian cell mutant to DNA minor groove ligands: lingandinduced DNA—protein crosslinking and responses to topoisomerase poisons + Paul J. Smith et al。 Carcinogenesis。 Vol 11 No. 4。 ―Multidrug Resistance Associated With Altered Topoisomerase II Activity—Topoisomerases II as Targets for Rational Drug Design‖ + Kenh Cowan amp。 Charles Marrow。 National Cancer Institute。 February 28, 1990. ―Mechanism of antitumor drug action: Poisoning of mammalian DNA topoisomerase II on DNA by 4‘(9acridinylamino)methanesulfonmanisidide‖ + Eric M. Nelson et al。 Proc. Natl. Acd. Sci. USA。 Vol 81。 Mar 1984。 ―Design of DNA Intercalators To Overe Topoisomerase IIMediated Multidrug Resistance‖ + Bruce C. Baguley et al。 Journal of the National Cancer Institute。 Vol 82。 Mar. 1990。
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