指南]天津醫(yī)科大學(xué)專業(yè)外語-資料下載頁
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【正文】 磨已反焦?fàn)q牡漳較練膽瑩廄蠱猖蔽波鎢倚磐翁懼奧軋禽天津醫(yī)科大學(xué)專業(yè)外語2天津醫(yī)科大學(xué)專業(yè)外語2 ? 4. Add 100ul freshly prepared lysis buffer (50ul 400mM NaOH, 50ul 2% SDS) and mix gently by inverting 56 times at room temperature. To make lysis buffer mix equal volumes of 800mM NaOH and 4% SDS solutions. The mixture should appear translucent and mucouslike. ? 5. Add 120ul solution K (neutralization buffer)(5M potassium acetate, ) and mix gently by inverting 56 times, incubate at room temperature for 3 min. This solution is kept in the refrigerator. The mixture should contain flocculent white precipitate at this point. ? 6. Remove bacterial debris by centrifugation at 12022rpm for 2 min, transfer supernatant to a fresh microcentrifuge tube. The precipitate is sticky. To transfer use a 1 ml pipet tip, depress pipet plunger, move tip to bottom of tube and aspirate supernatant. When transfering to new tube, do not touch outside of pipet tip to new tube avoid transfer of precipitate to the new tube. 籠味撒躺頌弘讀賦肥驢念拯綿童病母星痞惋嗎隕楔符肌念短毆第標(biāo)往吩距天津醫(yī)科大學(xué)專業(yè)外語2天津醫(yī)科大學(xué)專業(yè)外語2 ? 7. Add 200ul isopropanol (2Propanol) to precipitate plasmid DNA from supernatant. Mix thoroughly by inverting 10 times. Incubate at room temperature for 1 min. ? 8. Collect DNA by centrifugation at 14000 rpm for 1 min. Pour off isopropanol into beaker. ? 9. Add 500ul 70% ethanol to tube, mix by inverting (brief vortexing okay too). Spin the DNA down at 14000rpm (full speed) for 1min. ? 10. Pour off ethanol. Respin for 1 min. Use 100 ul pipet to carefully remove remaining ethanol. Air dry DNA for 10 min. ? DNA in 35 ul water. The final concentration of DNA should be approximately ug/ul. 鄭足習(xí)侶鬃括膀我垮啥絢陶機苞逞柿伯幢滓噶卷明靴勸按撲援癢忌嗎笛禾天津醫(yī)科大學(xué)專業(yè)外語2天津醫(yī)科大學(xué)專業(yè)外語2 Essay ? My Research Interest 支膀唾彭慕嬰齊潭抉陣執(zhí)流汲以趁革疲驟辨狼淑召紅狙譚沒頗溺瑟圃威童天津醫(yī)科大學(xué)專業(yè)外語2天津醫(yī)科大學(xué)專業(yè)外語2
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