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菊花的組織培養(yǎng)(1)(編輯修改稿)

2025-05-29 13:33 本頁面
 

【文章內容簡介】 of upper surface, and some root development has occured Shoots have developed over the surface of the explant, along with roots from the lower surface. The result is parable with the control medium 二、相關知識 No BAP BAP reduced to mg. l1 No NAA Poor shoot development and no roots. Extensive callus generation Poor shoot development and no roots NAA reduced to mg. l1 More balanced development of roots and shoots. However, undifferentiated callus is developing at the edges of the explant Profuse development of roots and a reduced number of shoots. Undifferentiated callus is developing at the edges of the explant 二、相關知識 NAA increased to mg. l1 Profuse development of roots over the explant upper and lower surface, and few shoots. Some callus No MS salts No sign of regeneration from explant at all. MS salts reduced to g. l1 Some root growth but reduced development of shoots MS salts increased to g. l1 Shoots developed on upper surface of explant. No roots or callus. 二、相關知識 2022/5/30 25 植物細胞的全能性 植物細胞具有該植物體全部遺傳的可能性,在一定條件下具有發(fā)育成完整植物體的潛在能力。 差異 :(1) 受精卵的全能性最高 (2) 受精卵分化后的細胞中,體細胞的全能性比生殖細胞的低。 潛在全能性的原因 : 基因表達的選擇性 科學研究表明,處于離體狀態(tài)的植物活細胞,在一定的營養(yǎng)物質、激素和其他外界條件的作用下,就可能表現(xiàn)出全能性,發(fā)育成完整的植株。人工條件下實現(xiàn)的這一過程,就是植物組織培養(yǎng)。 三、實驗原理 2022/5/30 26 植物細胞全能性的表達 脫分化( dedifferentiation):將來自已分化組織的已停止分裂的細胞從植物體部分的抑制
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