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waters公司的uplc-tqd培訓(xùn)資料ms_4_qp_hw_oper(編輯修改稿)

2025-03-20 13:19 本頁(yè)面
 

【文章內(nèi)容簡(jiǎn)介】 合物分析,可使用 ESCi復(fù)合源功能,同時(shí)獲得 ESI和APcI的數(shù)據(jù)。 169。2022 Waters Corporation 29 ESCi的調(diào)用 169。2022 Waters Corporation 30 ESCi的調(diào)用(續(xù)) 169。2022 Waters Corporation 31 碰撞誘導(dǎo)解離 (源內(nèi)- CID) I s o l a t i o nV a l v eC I D R e g i o n169。2022 Waters Corporation 32 A potential difference between the capillary and the sample cone causes ions to accelerate. They undergo ionmolecular reactions due to the short mean free path in this area. These collisions impart energy into the ions thereby causing fragmentation 碰撞誘導(dǎo)解離 Collision Induced Dissociation( CID) 169。2022 Waters Corporation 33 Extraction Cone 100 150 200 250 300 0 2,000 4,000 6,000 8,000 10,000 12,000 m/z Intensity Collision Induced Dissociation- CID Analysis of Sulfamethazine by Electrospray Sample Cone 30V NH N N CH 3 CH 3 NH 2 S O O Sulfamethazine Hexapole ionbridge [M+H] + 277 278 279 280 281 282 2,000 4,000 6,000 8,000 10,000 12,000 Parent Ion Region MW 278 m/z Positive ion mode, ESI, 2 ul/min, 50% MeOH:49% H2O:1% HOAc 169。2022 Waters Corporation 34 Collision induced molecular ion dissociation prior to mass analysis Extraction Cone NH N N CH 3 CH 3 NH 2 S O O Sulfamethazine Sample Cone 70V 100 150 200 250 300 0 2,000 4,000 6,000 8,000 10,000 m/z Intensity Hexapole ionbridge 優(yōu)異的 CID技術(shù)(可編程) Analysis of Sulfamethazine by Electrospray [M+H] + 183 184 185 186 187 188 2,000 4,000 6,000 8,000 10,000 Fragment Ion Region MW 186 m/z C6H8N3O2S 92 156 186 169。2022 Waters Corporation 35 源內(nèi) CID 20ng Flunitrazepam APCI Varying Cone Voltage180 200 220 240 260 280 300 320 340 360 380 400 420 440m/z0100%0100%0100%0100%0100%0100%flunitr1 168 () Scan AP+ flunitr2 168 () Scan AP+ flunitr3 168 (
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