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ent amplification: a general method for DNA fingerpringting. European Patent Application, Publ. No.: EP0534858, no. . ? Vos, P. et al. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23:44074414. AFLP AFLP的實(shí)驗(yàn)過(guò)程 AFLP Features of AFLP Markers ? Free of artifacts ? Comigration of nonallelic fragments occurs at extremely low level ? No prior knowledge of genome is needed ? Perfect replicability ? Small quantity of DNA ? High efficiency, more markers per primer, high polymorphism ? Segregation as Mendelian ? High accuracy ? Sample all genome AFLP Shortings of AFLP ? Dominant (415% codominant) ? Problem in homology of markers AFLP Applications of AFLP Markers ? Systematics ? Biodiversity ? Population structure, differentiation, Fst, gene flow, dispersal, outcrossing, introgression, hybridization clonal identification modes of reproduction ? Fingerpringting: individual i