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in each Petri. Statistical analysis was performed based on SPSS (version ) and SPSS . The data were analyzed through oneway analysis of variance (ANOVA) to determine the effect of treatments, and least significant difference (LSD) test were performed to determine the statistical significance of the differences between means of treatments. The figures were protracted based on SPSS (version ) too, for perfect the thesis, different Tables and Figures were used in the thesis.At the end of the study, it’s concluded: 1. The seed germination and seedling growth was inhibited significantly by the heavy metals (Cr、Pb) treatements, pared with the control. The inhibition increased with the increase of heavy metals concentration.2. Cd ( mg/l), Zn () of lower concentration could raise germination percentage in radish and shoot elongation in radish. And at the higher concentration (Cd ≥ mg/l, Zn ≥ ), these two indexes was inhibited, pared with the control.3. At the different concentration of heavy metals treatment, the effect of inhibition on radish root growth was was more accurate to measure the toxic of heavy metal pollutions.4. The total Chl content and Car content of radish decreased with the increase of heavy metals concentrations. The Car content decreased less than the Chl content, and so a decrease in the Chl (a + b)/Car ratio in parison with the control was confirmed. Generally the Chl b content slightly decreased more than the Chla content.5. The accumulation of radish was higher in the roots than in the cotyledons. There was a significant positive correlation between the accumulation and heavy metals concentration.6. When chromium and cobalt as synergist pollutant, at lower concentration of these two elements to radish, there was an antagonistic effect. And at higher concentration of these two elements to radish, there was a synergistic effect.7. When lead and mercury as synergist pollutant, the toxicity of lead on shoot growth to radish increased when mercury was added. 8. When cadmium and zinc as synergist pollutant, there was a synergistic effect on seed germination to radish these two elements. At lower concentration of these two elements to radish, there was an antagonistic effect on root and shoot growth.Key words: Heavy metal。7. 在鉛和汞復合處理時,汞的加入增加了鉛的毒害作用,鉛的加入,降低了汞的毒害作用。最后用乙醇定容至10 mL,搖勻。重金屬是造成全球作物產量減少的重要原因之一。重金屬在液體中移動和其相對遷移能力主要取決于氧化還原條件、PH和有機絡合物量等環(huán)境條件,在溶液中,溶液的離子強度和陽離子的飽和條件也會影響到重金屬的移動,環(huán)境中大部分毒性重金屬由于其非溶性而很難在維管植物中自由轉移。鋅是生物體內許多重要生物酶的組成元素,其在氮的循環(huán)、細胞的合成代謝、光合作用和生物生長素合成中起著非常重要的作用,再加上鋅也是生物界所必須的營養(yǎng)元素之一,所以其對于植物的影響具有種類異質性,比如其毒性作用的大小與植物種類的生長年齡、生長時期以及鋅本身是否可以直接被植物利用等因素有關。重金屬鋅、鐵、銅、錳等是植物生長不可缺少的微量元素,它們在很多生物酶的合成與代謝過程中起到非常重要的作用,而其它重金屬如鉛,鎘,砷,銫,鉻,鋁等雖然在生物學上并不是必須的,但如果它們超過一定濃度將會對生物產生極大的毒性效應。在沒有受污染的土壤中,重金屬大都以很少量存在。在含有重金屬鎘的物質中,硫酸鹽是最常見的,它的溶液也是具有毒性的,而水中鎘的來源主要分為天然源和人類生產活動源。許多研究表明,當鉛通過植物的根和莖進入植物體內后,將在植物體內大量積累,然而其積累的量主要取決于鉛本身的特性。特別是對土壤的污染,從而污染了生長在土壤上的植物。6H2O ( 132 mg/l), PbCl2 (、 mg/l), HgCl2 (、 、 mg/l) and ZnCl2 (、 mg/l)。稱取干樣(,),再分別加入1mL混合酸(65% HNO3:H2O2=4:1)溶解,然后用高壓鍋在120℃下消毒滅菌90分鐘。 it is dependent on the age, vegetation state of the plant as well as on zinc availability . Radish are widely growth around the world。 Chlorophyll。C. All metals were determined after mineralization. For mineralization, the dry samples ( g of roots and g of cotyledons) were dissolved in 5 mL of HNO3 (65%) and a H2O2 mixture prepared in the ratio 4:1. After 24 h each sample was autoclaved separately in a teflon container at the temperature 120176。6. 在鉻和鈷復合處理時,低濃度表現為拮抗作用,較高濃度時,表現為協(xié)同作用。用滴管吸取乙醇,將濾紙上的葉綠體色素全部洗入容量瓶中,直至濾紙和殘渣中無綠色為止。如今,隨著工業(yè)化、城市化的發(fā)展,污染物包括重金屬進入環(huán)境的數量越來越多,各種疾病的突發(fā)率都與水果和蔬菜的食用緊密相關。當然,重金屬的生物毒性是與其存在的形態(tài)是密不可分的,比如就汞、鉛、砷、鎘來說,當它們以陽離子形態(tài)存在時毒性會增加。另外一些重金屬對植物以及生長具有更大的毒性作用,包括砷、鈷、鋁、硒等。由于汞、鎘、鉻、 鉛、砷的強毒性使得它們在土壤重金屬污染中占有非常重要的地位,除此之外,鋅、銅、 鈷、鎳、硒和一些其它元素也因其對生物的毒性而特別被關注。 從全球范圍來看,重金屬污染被認為是最主要的環(huán)境污染之一。汞是毒性很大的污染物。土壤中的重金屬污染源有很多。在污染環(huán)境下生長的植物將含有重金屬以及其他污染物,這必將影響到人們的健康生活水平。本實驗所用蘿卜種子由甘肅省農科院提供。冷卻后用蒸餾水稀釋定容至25mL,最后用原子吸收分光光度計測定重金屬含量;試驗數據源于每組濃度六次重復的平均值,數據分析應用SPSS ()軟件,對數據進行單因素方差分析(oneway analysis of variance (ANOVA))和多重比較(least significant difference (LSD))。 the aim of the study was to pare the effect of Zn2+ to seed germination, root and plumue growth of radish. Lead is not essential for plants, and excessive amounts can cause growth inhibition, as well as reduced photosynthesis, mitosis, and water lead and dissoluble lead are poisonous, Pb can be entered the environment by mine, melting, pesticide, transportation, coal and industry related to Pb, Pb was regarded as no positive bioeffect, many experiment and analysis have proved that Pb can enter the tissue of plant through root and shoot, and then enriched in plant, and the enrich degree pared to it’s habitat. The source of heavy metal pollutant in soil have many approaches, firstly, some soil itself contain heavy metal inbeing, different soil type contain different heavy metal pollutant, in another, because of human’s activity, heavy metal pollution were spread out to the air, water and at trace levels heavy metals are natural ponents of soils, activities such as mining, industry, and localized intensive agriculture have contributed to undesirable accumulations of toxic metals in many areas around the world. Furthermore, heavy metal pollution has increased with the population density。 Metal accumulation。1℃ day/night temperature. The germination rate was recorded at different time, after germination, the root and shoot length were measured the sprout up to half of the seed length was regarded as germinated, according to the different seed and different heavy metals, the note time of different test were different too, at the end of the test, the root and shoot length of every seed were measured using Digital Caliper. All the data of the thesis were means of six replicated.The chlorophyll and the carotenoid content of plant were determined 7 days after the addition of the test solutions. Fresh leaves ( g) were homogenized using a pestle and mortar in to 95% ethanol or 80% aceton