【正文】 開發(fā)的目的和意義 ...........................................................................................................3 1．農(nóng)業(yè)植物疫病蟲害發(fā)生頻繁 ...............................................................................................3 2．茄科土傳病害危害及經(jīng)濟(jì)重要性 ........................................................................................3 3．茄科土傳病害流行猖獗 ......................................................................................................3 4．茄科土傳病害的危害及防治的關(guān)鍵問題 .............................................................................4 三、國內(nèi)外相關(guān)技術(shù)發(fā)展概況和趨勢 .............................................................................................5 （一）青枯病研究進(jìn)展 ...........................................................................................................5 1．植物青枯病的癥狀 ......................................................................................................5 2．青枯雷爾氏菌的形態(tài)特征 ...........................................................................................5 3．青枯病發(fā)病規(guī)律和侵染循環(huán) ........................................................................................6 4．植物青枯病的生物防治研究進(jìn)展 .................................................................................6 （二）枯萎病研究現(xiàn)狀 ...........................................................................................................8 1．枯萎病的發(fā)生與危害 ..................................................................................................8 2．枯萎病原菌的微生物學(xué)特性 ........................................................................................9 3．枯萎病原菌的生理生化 ............................................................................................. 11 4．枯萎病的防治 ........................................................................................................... 12 四、前期研制開發(fā)情況 ................................................................................................................ 18 （一）青枯雷爾氏菌及其生物防治研究基礎(chǔ) ......................................................................... 18 1．青枯雷爾氏菌致病力分化的研究 ............................................................................... 18 2． 青枯雷爾氏菌 強(qiáng)致病力菌株和無致病力菌株的鑒別 ................................................... 19 3．青枯病生物殺菌劑的研究基礎(chǔ) .................................................................................. 21 4．無致病力青枯雷爾菌的 GFP 基因標(biāo)記 ...................................................................... 22 （二）尖孢鐮刀菌及其生物防治研究基礎(chǔ) ............................................................................. 23 1．尖孢鐮刀菌的培養(yǎng)特性 ............................................................................................. 23 2．蔗糖濃度對尖孢鐮刀菌生長的影響 ........................................................................... 28 3．培養(yǎng)基營養(yǎng)成分對 尖孢鐮刀菌生長的影響 ................................................................. 30 4．尖孢鐮刀菌的生防因子 芽孢桿菌的初篩 .................................................................... 34 5．尖孢鐮刀菌生防因子芽孢桿菌的復(fù)篩 ........................................................................ 37 五、主要研制開發(fā)內(nèi)容和考核目標(biāo) .............................................................................................. 40 1．建設(shè)規(guī)模 ......................................................................................................................... 40 茄科土傳病害生物防治拮抗菌劑生產(chǎn)工藝關(guān)鍵技術(shù)的研究與應(yīng)用 1 2．研究內(nèi)容 ......................................................................................................................... 40 六、項(xiàng)目組織實(shí)施方案 ................................................................................................................ 42 1．項(xiàng)目的技術(shù)路線 .............................................................................................................. 42 2．項(xiàng)目建設(shè)期限與進(jìn)度安排 ................................................................................................ 43 3．研究隊(duì)伍人員配置 ........................................................................................................... 43 七、產(chǎn)業(yè)化依托或工程依托落實(shí)情況 ........................................................................................... 44 1．項(xiàng)目承擔(dān)單位 .................................................................................................................. 44 2．項(xiàng)目成果對接企業(yè) ........................................................................................................... 46 3．與企業(yè)的對接協(xié)議 ........................................................................................................... 46 八、技術(shù)經(jīng)濟(jì)分析和社會經(jīng)濟(jì)效益分析 ....................................................................................... 48 1．茄科土傳病害拮抗菌劑的研究為解決 “餐桌污染 ”提供技術(shù)保障 ........................................ 48 2．茄科土傳病害拮抗菌劑的應(yīng)用對提高中國農(nóng)產(chǎn)品貿(mào)易競爭力意義重大 .............................. 48 3．茄科土傳病害拮抗菌劑的研究與綠 色食品產(chǎn)業(yè)息息相關(guān) ................................................... 48 4．茄科土傳病害拮抗菌劑的研究是創(chuàng)匯農(nóng)業(yè)產(chǎn)業(yè)的基礎(chǔ) ...................................................... 48 5．茄科土傳病害拮抗菌劑的研究與環(huán)境保護(hù)產(chǎn)業(yè)緊密相聯(lián) ................................................... 48 6．茄科土傳病害拮抗菌劑的研究將成為我省生物工程產(chǎn)業(yè)龍頭 ............................................ 49 7．茄科土傳病害拮抗菌劑的應(yīng)用將帶動(dòng)商業(yè)貿(mào)易的發(fā)展 ...................................................... 49 九、項(xiàng)目資金預(yù)算 ....................................................................................................................... 50 1．資金預(yù)算 ......................................................................................................................... 50 2．資金籌措 ......................................................................................................................... 50 十、項(xiàng)目申報(bào)單位及協(xié)作單位概況 .............................................................................................. 51 十 一、其他需要說明的情況 ......................................................................................................... 52 1．安全食品的發(fā)展對本研究的需求 ..............................................................