科大高分子課件四聚合物在生物高分子分離中的應(yīng)用(存儲(chǔ)版)
【正文】 ontaining LPA with different molecular masses Polymer Mv of LPA (MDa) Intrinsic viscosity of LPA (mL/g) Molar ratio of AM/DMA Intrinsic viscosity of IPN (mL/g) quasiIPN 1 106 :1 88 quasiIPN 2 106 :1 145 quasiIPN 3 168 :1 167 quasiIPN 4 168 :1 266 quasiIPN 5 278 :1 260 quasiIPN 6 928 :1 840 0 1 2 3 4 5 6 7 0 1 2 3 4 5 6 7 0 1 2 3 4 5 6 731Mi g r a ti on ti m e ( m in )Bare ca p il lary0. 01AU2321Mi g r a ti on ti m e ( m in )Co a ti n g321Mi g r a ti on ti m e ( m in )S e ivin g EOF as a function of pH. Electropherograms of a mixture of standard proteins. 0 2 4 6 8 10 12 14 1631 M i gra t i on t i me ( mi n)Absorbance321 2321 321 p H 3p H 4p H 5p H 612 14 16 18 20 22 24 26 28 30 3212 14 16 18 20 22 24 26 28 30 3212 14 16 18 20 22 24 26 28 30 3212 14 16 18 20 22 24 26 28 30 32d Mi g r a ti o n ti m e ( m in)c1353107887260331028127123419411872 b Intensity aSeparation of Φ 174/HaeIII digest by CE with quasiIPN3 at (a) 2% w/v。 。 (c) % w/v 0 2 4 6 8 10 12 14 1631 M i gra t i on t i me ( mi n)Absorbance321 2321 321 p H 3p H 4p H 5p H 6 Effect of pH values on separations of basic proteins using HECgPDMA coated , lysozyme。 temperature 25℃ . Effect of buffer pH on the separation of basic proteins. separations were taken in PEO113bP4VP294 coated capillary Electropherograms of plasma sample in bare capillary (A) and a PEO113bP4VP294 coated capillary (B). Separation buffer: 19mM NaOHNa2B4O7 at pH . HSA, human serum albumin。 3 = Ribonuclease A. Migration time reproducibility (n = 3) and peak efficiency of p
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