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畢業(yè)論文-鐵離子和鈣離子對(duì)杏鮑菇菌絲生長的影響研究(終稿)-免費(fèi)閱讀

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【正文】 突然想到那件藍(lán)格子襯衫;那些被折成各種各樣形狀的信紙;那段從街角深巷伊始的友誼;還有那場還沒有開始就宣告了終結(jié)的邂逅計(jì)劃……那些年那些天的非做不可,終于和青春一樣,都將在我們的人生中謝幕。也曾經(jīng)好像已經(jīng)下定了決心去做某件事,但更多的時(shí)候是最后又打起了退堂鼓。t really grow up, it seems is not so important。s actions.CSKA said they were surprised and disappointed by Toure39。s 150th anniversary celebrations and will attend City39。t charge you more than 35% of your pensation your employer dismisses you and you think that you have been dismissed unfairly.For more information about dismissal and unfair dismissal, see 四年來,系里全體領(lǐng)導(dǎo)和老師的無私奉獻(xiàn)和諄諄教導(dǎo),給了我巨大的幫助,照亮了我前行的方向,這也將是我人生的一筆重要財(cái)富。其次,本文只是在PDA培養(yǎng)基中研究不同濃度的金屬離子對(duì)杏鮑菇菌絲的生長影響,而對(duì)于袋裝工業(yè)化子實(shí)體生長過程中添加鐵,鈣離子是否有相同的影響,也有待于更深一步實(shí)驗(yàn)。培養(yǎng)20天后,結(jié)束培養(yǎng)。配制PDA培養(yǎng)基:馬鈴薯(去皮)200g加水1000ml煮沸15~20min后,用4層紗布過濾將濾液煮沸加入瓊脂20g,葡萄糖20g,補(bǔ)足無菌水至1000ml。試管分別貼好標(biāo)簽,高壓蒸汽滅菌。 菌種的活化活化菌種培養(yǎng)皿的制作配制PDA培養(yǎng)基:馬鈴薯(去皮)200g加水1000ml煮沸15~20min后,用4層紗布過濾將濾液煮沸加入瓊脂20g,葡萄糖20g,補(bǔ)足無菌水至1000ml,調(diào)節(jié)PH至5~,趁熱分裝至試管中,每管15ml,進(jìn)行加塞包扎,高壓蒸汽滅菌。 菌種 杏鮑菇母種 湘南學(xué)院化學(xué)與生命科學(xué)系微生物實(shí)驗(yàn)室提供 實(shí)驗(yàn)方法 菌種的純化 斜面培養(yǎng)基制作配制PDA培養(yǎng)基:馬鈴薯(去皮)200g加水1000ml煮沸15~20min后,用4層紗布過濾將濾液煮沸加入瓊脂20g,葡萄糖20g,補(bǔ)足無菌水至1000ml,調(diào)節(jié)PH至5~,趁熱分裝至試管中,每管10ml,進(jìn)行加塞包扎。鈣是一些重要酶類的活化劑[7]。葉子中植物鐵蛋白儲(chǔ)存作為形成質(zhì)體用,是進(jìn)行光合作用所必需的[6]。礦物元素也很豐富,人體必需的微量元素鈣和鎂的含量特別高。長期以來, 杏鮑菇在全世界鮮有商業(yè)化栽培。杏鮑菇子實(shí)體單生或群生,菌蓋初球形、半球形,后漸平展,淺黃白色。屆 別 2011 學(xué) 號(hào) 200714250126 畢業(yè)設(shè)計(jì)(論文)鐵離子和鈣離子對(duì)杏鮑菇菌絲生長的影響研究 目 錄摘 要 IABSTRACT II1 引言 1 杏鮑菇 1 金屬元素 1 鐵元素在生物體中的作用 2 鈣元素在生物體中的作用 2 選題的意義 22 實(shí)驗(yàn)部分 2 主要儀器與試劑 3 主要儀器 3 基本試劑和藥品 3 主要的玻璃儀器及其他實(shí)驗(yàn)用具 3 菌種 3 實(shí)驗(yàn)方法 3 菌種的純化 3 菌種的活化 4 鐵元素對(duì)杏鮑菇菌絲生長的影響 4 鈣元素對(duì)杏鮑菇菌絲生長的影響 63. 實(shí)驗(yàn)結(jié)果與分析 9 9 9 討論 10參考文獻(xiàn) 12致謝 13摘 要通過配制一系列不同F(xiàn)e,Ca離子濃度的固體培養(yǎng)基培養(yǎng)杏鮑菇菌絲,觀察其生長情況,得出微量元素對(duì)杏鮑菇菌絲生長的最適濃度。菌褶延生,密集,乳白色。近年, 泰國、美國、日本、我國的臺(tái)灣省采用調(diào)溫、調(diào)濕的自動(dòng)化生產(chǎn)工藝進(jìn)行試驗(yàn)性栽培, 取得成功[3]。此外,還含有銅、鋅、錳、鐵、鉀、磷等元素,膳食中多食用杏鮑菇可提高人體免疫功能,有抗癌、降血脂、潤腸胃、美容等功效,亦可促進(jìn)人體對(duì)脂類物質(zhì)的消化吸收和膽固醇的溶解,是老年人和心血管疾病下肥胖癥患者理想的營養(yǎng)保健品[5]。鐵是鉬2鐵蛋白和鐵蛋白的組分,固氮酶缺鐵就沒有固氮活性。鈣可促進(jìn)硝態(tài)氮吸收, 與氮代謝有關(guān), 它有助于減少植物中的硝酸鹽, 中和植物中的有機(jī)酸, 對(duì)代謝過程中產(chǎn)生的有機(jī)酸有解毒作用。高壓蒸汽滅菌:首先將內(nèi)層鍋取出,再向外層鍋內(nèi)加入適量的水,使水面與三角擱架相平為宜。將用于培養(yǎng)用的培養(yǎng)皿用報(bào)紙包好,干熱滅菌。在無菌操作下,在超凈工作臺(tái)內(nèi)將滅好菌的試管冷卻至50~60℃時(shí)倒入干熱滅菌冷卻后的培養(yǎng)皿內(nèi),貼好標(biāo)簽,冷卻靜置待用。不同濃度鈣元素培養(yǎng)基的制作:分別用移液管取1ml各濃度的氯化鈣溶液和蒸餾水于貼好標(biāo)簽的燒杯中,加培養(yǎng)基至50ml,調(diào)pH值5~。由測得的數(shù)據(jù)可以分別求出每組培養(yǎng)皿在不同培養(yǎng)時(shí)期時(shí)的平均直徑,并填入表內(nèi),可以得出:在一定范圍內(nèi),不同濃度的鈣離子加入PDA培養(yǎng)基內(nèi)都可以使杏鮑菇菌絲菌落直徑增大,當(dāng)鐵離子濃度為350ug/ml時(shí),這種作用效果最明顯,當(dāng)超過這個(gè)濃度時(shí)作用效果變?nèi)?。最后,?dāng)人類食用了經(jīng)過添加鐵,鈣離子的培養(yǎng)基培養(yǎng)出來的杏鮑菇后,是否會(huì)產(chǎn)生不良影響,這也是人們最關(guān)注的問題。我每一次小小的進(jìn)步,都傾注了老師們的心血。Dismissal.You can make a claim to an employment tribunal, even if you haven39。if you win the case.If you are thinking about signing up for s Premier League match at Chelsea on Sunday.I am going to be at the match tomorrow and I have asked to meet Yaya Toure, he told BBC Sport.For me it39。s plaint. In a statement the Russian side added: We found no racist insults from fans of CSKA.Age has reached the end of the beginning of a word. May be guilty in his seems to passing a lot of different life became the appearance of the same day。 Then in high school, think don39。嫌惡過自己的怯懦,最終卻發(fā)現(xiàn)有很多緣分,有很多錯(cuò)過,好像冥冥之中真的已經(jīng)注定。Baumgartner the disappointing news: Mission aborted. r plays an important role in this mission. Starting at the ground, conditions have to be very calm winds less than 2 mph, with no precipitation or humidity and limited cloud cover. The balloon, with capsule attached, will move through the lower level of the atmosphere (the troposphere) where our daytoday weather lives. It will climb higher than the tip of Mount Everest ( miles/ kilometers), drifting even higher than the cruising altitude of mercial airliners ( miles/ kilometers) and into the stratosphere. As he crosses the boundary layer (called the tropopause),e can expect a lot of turbulence.The supersonic descent could happen as early as Sunda.The weatheThe balloon will slowly drift to the edge of space at 120,000 feet ( Then, I would assume, he will slowly step out onto something resembling an Olympic diving platform.Below, the Earth bees the concrete bottom of a swimming pool that he wants to land on, but not too hard. Still, he39。t start planning... Those years, those days of do, finally, like youth, will end in our life.  此刻,天空是陰暗的,空氣里有著剛下過雨之后的清新因子。想要的,不想要的,界限明確,好像沒有什么可以撼動(dòng)自己。 Junior high school, thought to have a crush on just means that the real growth, but over the past three years later, his writing of alumni in peace, suddenly found that isn39。s governing body, has also ordered an immediate investigation
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