【正文】 310 人參、五靈脂和人參配伍的總皂苷含量 ................................. 14 表 311 樣品稱量 ........................................................... 15 表 312 人參皂苷檢測的色譜條件 ............................................. 16 表 313 含量測定結(jié)果 ....................................................... 19 表 41五靈脂各層提取物清除 關(guān)鍵詞：五靈脂，人參，化學(xué)成分，配伍實驗，抗氧化活性，抑菌活性 III Study on patibility of trogopterus dung and panax ginseng changes in chemical and pharmacological activity in vitro Abstract Trogopterus dung and Panax ginseng, is one pair of nieen herbs of mutual antagonism. Was prescription in mon use in clinical practice. Experiments on patibility of the tow medicines were practiced and summarized in this thesis. Chemical and pharmacology studies are tow main parts of the thesis. In chemical works, tow pounds were isolated from EtOAc fraction of trogopterus dung abstract they were Beta sitosterol and protocatechuic acid. Three pounds were isolated from Ginseng abstract, they were saponin Rd, Rc and Rb1. Fatsoluble position in trogopterus dung was analysed by GCMS method, 42 pounds were identified and 15 of them were fatacid, with a content of percent. Volatile oil in trogopterus dung was extracted by steam distillation. Four monly used patibility proportion of the tow medicines were pared, 5:1, 3:1, 1:1 and 1:3. Variation of content of total flavonoid and saponin were determined by UV, while content of protocatechuric acid and other nine saponins were determine by HPLC. Results showed under polypharmacy condition, saponin in ginseng declined except Rb3, and there was no linear relationship between proportion and the content. Content of protocatechuric acid varied as change of patibility proportion, under condition of 5:1, 3:1 and 1:1, protocatechuric acid in extract had a higher content than in single trogopterus dung. In pharmacology study, antioxidation activity was tested by DPPH method. Activity of extracts from trogopterus dung by different solvent, volatile oil, and different patibility proportion were pared, EtOAc and nBuOH fraction of trogopterus dung had stronger activity in eliminating DPPH radical, and the activity decreased as gingseng goes to a higher proportion in patibility. Bacteriostatic study was carried on colibacillus and staphylococcus aureus, all above groups performed inhibition, chloroform and ethyl acetate extract of trogopterus dung showed outstanding inhibition ratio, under 25 mg/ml condition, the ratio came to % and %. Volatile oil of trogopterus dung also showed inhibition activity, but was not significant. Both gingseng and trogopterus dung ethanol extract were able to inhibit colibacillus and staphylococcus aureus, while trogopterus dung group performed stronger. Proportion 5:1 came to the highest inhibition ratio at concentration of 25 mg/ml, the ratio was % and %, which was significant difference to the blank, that was even higher than single trogopterus dung. IV Our study manifested the following results: Compatibility of the tow medicines, affected content of some saponin in ginseng, and enhanced activity ponents in trogopterus dung. As proportion of panax ginseng went higher, activity of antioxidation and bacteriostasis declined, but some of them performed stronger. This indicates that, patibility of trogopterus dung and panax ginseng is feasible in clinical use for treatment of trogopterus dungtreating diseases. Key word： Trogopterus dung， Panax ginseng， Chemical studies ， patibility，antioxidation activity， Bacteriostatic study 目 錄 第一章 前言 ................................................................. 1 五靈脂與人參配伍研究概況 ............................................ 1 五靈脂研究概況 ...................................................... 5 人參研究概況 ........................................................ 8 第二章 五靈脂、人參化學(xué)成分研究 ............................................ 10 五靈脂的化學(xué)成分研究 ............................................... 10 人參的化學(xué)成分研究 ................................................. 19 第三章 五靈脂與人參配伍化學(xué)成分變化研究 .................................... 22 五靈脂與人參配伍中總黃酮的含量變化研究 ............................. 22 五靈脂與人參配伍的原兒茶酸含量變化研究 .............................. 4 人參與五靈脂配伍中總皂苷的含量變化研究 ............................. 11 人參與五靈脂配伍中 9 個人參皂苷單體的含量變化研究 ................... 15 第四章 ˙ DPPH 法體外抗氧化活性研究 ......................................... 23 清除 DPPH 自由基能力比人參更好，合用后， 隨著五靈脂量的減少，添加人參量的增加，其清除 在藥理實驗方面主要進行了 以 下研究： ，本論文 采用 DPPH 法， 對五靈脂 不同溶劑 提取物及其揮發(fā)油、五靈脂與人參不同比例配伍后的醇提物進行體外抗氧化活性研究，結(jié)果表明 ：各組分均對 ，選取了 4個 應(yīng)用較廣泛的配伍比例，分別為：五靈脂與人參配伍 5: 3: 1: 1:3。 本論文包括化學(xué)實驗研究和藥理實驗研究兩大部分 。 學(xué)位論文作者簽名： 簽字日期： 年 月 日 關(guān)于論文使用授權(quán)的聲明 本人完全了解吉林 農(nóng)業(yè)大學(xué)有關(guān)保留和使用學(xué)位論文的規(guī)定，即：研究 生在校攻讀學(xué)位期間所完成的論文及相關(guān)成果的知識產(chǎn)權(quán)屬吉林農(nóng)業(yè)大學(xué)所有，并同意將本論文的版權(quán)授權(quán)給吉林農(nóng)業(yè)大學(xué)，學(xué)校有權(quán)保留送交論文的復(fù)印件和磁盤，允許論文被查閱和借閱，可以采用影印、縮印或掃描等復(fù)制手段保存、匯編學(xué)位論文。 五靈脂與人參配伍化學(xué)成分及體外藥理活性變化 研究 碩士（博士）學(xué)位論文 五靈脂與人參配伍化學(xué)成分及體外藥理活性變化 研究 Study on patibility of trogopterus dung and panax ginseng changes in chemical and pharmacological activity in vitro 2020 年 5 月 獨 創(chuàng) 性 聲 明 本人鄭重聲明：所呈交的學(xué)位論文是本人在導(dǎo)師指 導(dǎo)下，獨立進行研究所取 得的成果。 本學(xué)位論文所有內(nèi)容若有不實之處，本人愿意承擔(dān)一切相關(guān)法律責(zé)任和后果。 學(xué)位論文作者簽名： 簽字日期： 年 月 日 導(dǎo)師簽名： 簽字日期： 年 月 日 I 五靈脂與人參配伍化學(xué)成分及體外藥理活性變化研究 摘 要 五靈脂與人參，是中藥十九畏中臨床應(yīng)用較多的一對配伍，本論文對五靈脂與人參配伍進行了實驗研究，可為臨床合理配伍提供科學(xué)有效的重要依據(jù)。本論文還采用水蒸氣蒸餾法提取了五靈脂的揮發(fā)油。 ③ 對原兒茶酸的含量測定表明：配伍后溶液中的原兒茶酸含量變化顯著，且 五靈脂與人參配伍為 5:1， 3:1， 1:1時，溶液中的原兒茶酸含量都明顯高于單味五靈脂中的原兒茶酸含量。 DPPH 有較 強的清除能力 ； 五靈脂的清除 五靈脂不同溶劑提取物中，氯仿提取物和乙酸乙酯提取物對兩種菌的抑菌效果都尤為顯著，其中在氯仿提取物濃度為 25 mg/ml 時，對兩種菌 的 抑制率都最高，達到 %和%，與空白對照組比較有極顯