【正文】 ...7 3. 實驗方法及原理 .............................................................................................................. 7 降解丙烯酰胺菌篩選流程 ...........................................................................................................................7 菌株的富集 ...................................................................................................................................................7 菌株的分離純化 ...........................................................................................................................................8 菌種的生理生化鑒定 [45] .............................................................................................................................8 革蘭氏染色 ...........................................................................................................................................8 接觸酶試驗 ...........................................................................................................................................8 甲基紅試驗 ...........................................................................................................................................8 VP 試驗 .................................................................................................................................................8 葡萄糖氧化發(fā)酵實驗 ...........................................................................................................................9 重絡(luò)酸鉀法測 COD【 68】 ..............................................................................................................................9 丙烯酰胺降解率的測定 ...............................................................................................................................9 丙烯酰胺含量的測定 ................................................................................................................................ 10 4. 結(jié)果與分析 .................................................................................................................... 11 降解丙烯酰胺菌的分離 .............................................................................................................................11 優(yōu)勢菌的菌體染色及形態(tài)鑒定 ................................................................................................................11 II 菌體染色 .............................................................................................................................................11 生理生化鑒定 .................................................................................................................................... 12 菌株生長條件優(yōu)化 ..................................................................................................................................... 12 降解時間的影響 ................................................................................................................................. 12 接種量的影響 .................................................................................................................................... 13 培養(yǎng)溫度的影響 ................................................................................................................................. 13 初始 pH 值的影響 .............................................................................................................................. 14 污水 COD 值測定 ...................................................................................................................................... 14 菌株對污水 COD 降解結(jié)果 ............................................................................................................. 15 菌株對丙烯酰胺除去率結(jié)果 ........................................................................................................... 16 .........................................................................................