馬鈴薯離體塊繁的實(shí)踐與應(yīng)用(文件)
【正文】 of high temperature (38℃ , 2 weeks) for virusfree 莖尖培養(yǎng)脫毒 原理 ? 病毒的分布不均勻 ， 越靠近莖頂?shù)母腥驹捷p ， 生長(zhǎng)點(diǎn) （ 約 － ） 幾乎不含或含病毒很少 。 Initiation of culture 1 stage Purpose Establishment of aseptic cultures in vitro Operation Take explants from mother plants such as buds leaf pieces, flowers… into the vessels. Sterile Explant Put on medium Wash with steriled water Take bud under aseptic conditions ? Establishment of aseptic cultures in vitro ? ? Medium ? ? Culture environment conditions? Discussions Stage 1 1. Wash the potato shoot tips for 10 min. 2. Soak them in 70% ethanol solution for 30 sec. 3. Soak them in 5% NaClO or % HgCl solution for 15 or 10 min. for surface sterilization. 4. Rinse them at least 35 times with sterile water 次亜塩素酸水溶液 5％に 15分間浸漬 滅菌水で3回以上 すすぐ 70％エタノールに30秒間浸漬 ％ ルStage 1 6. Excise a meristem () from a shoot tip 7. Place it aseptically on sterilized medium Medium with sucrose 30 g L1 meristem ? MS+ +3%蔗糖 ? 溫度 ： 21~25℃ ? 光照度 ： 20223000 lx
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