纖維素酶輔助提取仙鶴草中總黃酮的研究畢業(yè)論文-wenkub
2022-09-06 21:49:30 本頁面
【正文】 雄師范學(xué)院本科論文（設(shè)計(jì)） II Enzyme assisted extraction of total flavonoids from Agrimonia pilosa Abstract： Using enzyme hydrolysis and ethanol extraction bined methods of extracting total flavone from agrimony, separately from the enzymolysis temperature, enzymolysis time, enzymolysis solution pH, enzyme concentration to examine the extraction conditions on agrimony total flavone extraction rate in effect, and the orthogonal experiment, using chromogenic reaction of qualitative identification, using rutin as the standard control sample determination of total flavonoids in Herba agrimoniae, extraction rate. The results show that, using 70% ethanol as extraction solvent, using the enzyme alcohol extraction of total flavonoids from it: the optimal conditions for enzymatic hydrolysis temperature of 50 degrees C, enzymolysis time2h, enzyme solution pH value 5, enzyme concentration / mL. Under this condition, agrimony total flavone extraction rate in up to %. . Keywords： Cellulase。 楚 雄 師 范 學(xué) 院 本 科 生 畢 業(yè) 論 文 題 目： 纖維素酶輔助提取仙鶴草中總黃酮的研究 專 業(yè)： 化學(xué) 姓 名： 劉婭萍 學(xué) 號： 20202051242 指導(dǎo)教師： 宋建梅 職 稱： 副教授 論文字?jǐn)?shù)： 6653 字 完成日期： 2020 年 5 月 教 務(wù) 處 印 制楚雄師范學(xué)院本科論文（設(shè)計(jì)） 目錄 摘要 ..................................................................................................................................I 關(guān)鍵詞 ..............................................................................................................................I Abstract.......................................................................................................................... II Keywords ....................................................................................................................... II 前言 ................................................................................................................................. 1 1 實(shí)驗(yàn)部分 ...................................................................................................................... 2 原料、儀器和試劑 ...................................................................................................... 2 原料 ......................................................................................................................... 2 試劑 ......................................................................................................................... 2 儀器 ......................................................................................................................... 2 實(shí)驗(yàn)方法 .................................................................................................................... 2 原料處理 .................................................................................................................. 2 黃酮的提取 .............................................................................................................. 2 黃酮的定性鑒定 ....................................................................................................... 2 仙鶴草 中總黃酮提取率的計(jì)算 .................................................................................. 3 蘆丁標(biāo)準(zhǔn)溶液的配制 .........................................................................................
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