【正文】 畢業(yè)論文不同親本數(shù)目的中間球海膽群體遺傳結構分析目 錄摘 要................................................................ IAbstract............................................................... II第一章 前 言....................................................... 1第二章 材料與方法.................................................. 3 實驗材料..................................................... 3 實驗方法..................................................... 3 DNA提取............................................... 3 PCR擴增................................................ 3 數(shù)據(jù)統(tǒng)計與分析...............................................5第三章 實驗結果..................................................... 6 微衛(wèi)星擴增位點分析..........................................6 各位點等位基因情況..........................................7 遺傳多樣性分析...............................................9第四章 討 論...................................................... 10 海膽幼蟲DNA提取方法的優(yōu)勢...............................10 海膽遺傳學研究現(xiàn)狀......................................... 10致 謝................................................................12參考文獻............................................................. 13摘 要本實驗利用10對微衛(wèi)星引物，對由不同親本數(shù)目所產(chǎn)生的中間球海膽（Strongylocentrotus intermedius）群體進行了遺傳結構分析。實驗所用的兩個海膽群體分別來源于7個（命名為P7）和3個親本(命名為P3)，P7群體取100只個體，P3群體取樣50只。所有海膽稚膽期采用直接裂解法提取基因組DNA，裂解液直接進行微衛(wèi)星擴增。結果表明：P3群體擴增出24個等位基因，177。，177。，177。，P7群體擴增出24個等位基因，177。，177。，177。；所有遺傳學指標在兩個群體間沒有顯著差異。該研究首次建立了中間球海膽稚膽的固定、DNA提取方法并對結果進行了PCR擴增驗證，研究結果豐富了中間球海膽分子遺傳學內(nèi)容。關鍵詞：中間球海膽，親本數(shù)目，遺傳學，微衛(wèi)星Abstract10 pairs of microsatellite primers were used to analyze the genetic structure of the two Strongylocentrotus intermedius populations which were produced by different parental numbers. 100 individuals derived from 7 parents (named P7) and 50 from 3 parents (named P3) were subjected to microsatellite analysis. DNA of juvenile urchins was isolated from direct lysis and the lysis buffer were directly amplified. Results showed that: 24 alleles in total were detected in P3 population, the means of effective number of alleles, Shannon’s index and Nei39。s expected heterozygosis were 177。, 177。 and 177。. 24 alleles were detected in P7 population, the means of effective number of alleles, Shannon’s index and Nei39。s expected heterozygosis were 177。, 177。 and 177。. All of the genetic indexes between the two groups were not significantly different. This research was the first try of tissue fixation, DNA isolation and PCR amplification of juvenile sea urchin, and the results enriched the content of molecular genetics of sea urchin.Key words: Strongylocentrotus intermedius, parental stock size, genetics, microsatellites第一章 前 言