【正文】 SHANDONGＵＮＩＶＥＲＳＩＴＹ?。希啤。裕牛茫龋危希蹋希牵? 畢 業(yè) 論 文 玉米芯誘導(dǎo)里氏木霉合成纖維素酶學(xué) 院 ： 專 業(yè) 班 級 ： 學(xué) 生 姓 名 ： 學(xué) 號： 指 導(dǎo) 教 師 ： 201 年 月摘要I摘要 纖維素類物質(zhì)是自然界中最具潛力的一種可再生資源，其有效利用是目前人們關(guān)注的熱點(diǎn)。作為降解纖維素的多組分酶系，纖維素酶降解纖維素特異性高，反應(yīng)條件溫和，環(huán)境污染小。但由于其組分復(fù)雜，合成代謝網(wǎng)絡(luò)研究尚不清楚。此外，真菌纖維素酶多數(shù)是誘導(dǎo)酶，其合成須在誘導(dǎo)物存在下才能進(jìn)行，通過添加誘導(dǎo)物及對纖維素酶合成途徑進(jìn)行調(diào)控是提高纖維素酶活性的有效方法。因此，研究以天然纖維素物質(zhì)為底物的纖維素酶合成過程機(jī)理，充分挖掘現(xiàn)有生產(chǎn)菌株產(chǎn)纖維素酶的潛力，是解決纖維素酶誘導(dǎo)合成問題的關(guān)鍵。本文對天然纖維素物質(zhì)玉米芯誘導(dǎo)里氏木霉產(chǎn)纖維素酶的過程進(jìn)行了分析，通過測定玉米芯酶解液中糖的組分，并以所測定到的組分為誘導(dǎo)物進(jìn)行誘導(dǎo)產(chǎn)酶，發(fā)現(xiàn)除纖維素成分外，半纖維素水解產(chǎn)物半乳糖對纖維素酶的合成有誘導(dǎo)作用。通過優(yōu)化半乳糖的誘導(dǎo)濃度，發(fā)現(xiàn) 5g/L 的半乳糖對纖維素酶的誘導(dǎo)最好。關(guān)鍵詞：里氏木霉、纖維素酶、玉米芯、誘導(dǎo)AbstractIIAbstractCellulose is a natural substance in the most potential as a renewable resource, and its efficient use is currently the focus of attention. As a multiponent enzymes degrade cellulose, cellulose degradation of cellulose high specificity, mild reaction conditions, environmental pollution. However, due to the plexity of its ponents, anabolic Network Research unclear. In addition, most of the fungal cellulase enzyme induction, induction of the synthesis must be carried out under the presence, and by adding the inducer of cellulase synthesis pathway to improve the cellulase activity regulating effective way. Therefore, the study of natural cellulosic materials as substrates cellulase synthesis mechanism to fully exploit the existing strains producing cellulase production potential is to solve the problem of liquid fermentation of cellulase key.In this paper, natural cellulose substances corncob induced Trichoderma reesei cellulase production process was analyzed by measuring the sugar in corn cob hydrolyzate ponents, and the group to which the measured inducer for induction into production enzymes, found that in addition cellulose ingredients, hemicellulose hydrolyzate galactose on cellulase synthesis induced. By optimizing the concentration of galactose induction, found 5g / L of galactose induction of cellulase best.Key words: Trichoderma reesei, cellulase, corn cob, inducing目錄III目錄摘要 ................................................................................................................................IABSTRACT ..................................................................................................................II目錄 ..............................................................................................................................III第一章 緒論 研究進(jìn)展，立項(xiàng)依據(jù) ......................................................................1 里氏木霉簡介 ...................................................................................................1 木質(zhì)纖維素原料的組成 ...................................................................................1 纖維素酶研究進(jìn)展 ..........................................................................................2 纖維素酶的性質(zhì)和種類 .........................................................................3 纖維素酶的作用機(jī)理 ............................................................................4 纖維素酶的來源 .....................................................................................4 纖維素酶的應(yīng)用 ....................................................................................5 里氏木霉產(chǎn)纖維素酶的誘導(dǎo)物 ......................................................................6 纖維素 ....................................................................................................6 槐糖 ........................................................................................................7 乳糖 ........................................................................................................7 纖維二糖 ................................................................................................7 立項(xiàng)依據(jù) ..........................................................................................................7 本文研究問題 ..................................................................................................8第二章 材料方法 ......................................................................................................10 菌種 ................................................................................................................10 主要實(shí)驗(yàn)儀器 ................................................................................................10 實(shí)驗(yàn)藥品 ........................................................................................................11 實(shí)驗(yàn)試劑 ........................................................................................................12 檸檬酸緩沖液 .......................................................................................12 DNS 反應(yīng)液 ..........................................................................................12 微量元素 ...............................................................................................12 實(shí)驗(yàn)培養(yǎng)基 ..................................................................................................12 PDA 培養(yǎng)基 ..........................................................................................12 菌絲培養(yǎng)基（G/L） ...........................................................................13 誘導(dǎo)培養(yǎng)基（G/L） ...........................................................................13 實(shí)驗(yàn)方法： .....................................................................................................13目錄IV 預(yù)培養(yǎng) ...................................................................................................13 菌絲的獲取 ..........................................................................................13 取樣 .......................................................................................................14 酶活的定義及測定方法 .......................................................................14第三章 結(jié)果與討論 ................................................................................................18 玉米芯酶解液中糖組分的分析 .....................................................................18 不同誘導(dǎo)物誘導(dǎo)產(chǎn)濾