多次移植骨髓基質(zhì)干細胞有利于脊髓損傷修復(已修改)
2025-01-27 02:42 本頁面
【正文】 KKME專業(yè)醫(yī)學搜索引擎多次移植骨髓基質(zhì)干細胞有利于脊髓損傷修復作者：李輝映 文益民 陳克明 藍旭 汪大彬 孫振剛 胡凌云 作者單位：, 蘭州 730018； 。, 蘭州 730050； 基金項目：全軍醫(yī)學科學技術(shù)研究十一五計劃課題項目（編號：06MA081） 【摘要】 ［目的］研究多次經(jīng)蛛網(wǎng)膜下腔移植骨髓基質(zhì)干細胞對Wistar大鼠脊髓損傷的功能修復作用。［方法］骨髓基質(zhì)干細胞經(jīng)體外分離、培養(yǎng)并用Hoecst33342標記。按照Allen方法把60只在T10T12平面損傷的Wistar大鼠隨機分4組，A、B、C、D組（對照組）。在損傷平面蛛網(wǎng)膜下腔中段放置一硅膠管，在7 d后，注入1106個骨髓基質(zhì)干細胞。在12周熒光顯微鏡、免疫組化檢測骨髓基質(zhì)干細胞在損傷段脊髓的存活、分布、分化情況并作計數(shù)觀察。使用BBB評分觀測后肢功能恢復。［結(jié)果］移植后7～14 d骨髓基質(zhì)干細胞達到高峰，在7 d后表達巢蛋白及神經(jīng)絲蛋白陽性。隨時間的延長，移植在損傷部位的骨髓基質(zhì)干細胞數(shù)量及神經(jīng)元樣細胞均有減少，但移植3次的細胞數(shù)減少速度較其他兩組慢。移植3次組大鼠的BBB評分較移植2次組有明顯的提高，P＜,有統(tǒng)計學意義。［結(jié)論］多次移植骨髓基質(zhì)干細胞更有利于脊髓損傷的恢復。 【關(guān)鍵詞】 脊髓損傷 骨髓間充質(zhì)干細胞 移植 蛛網(wǎng)膜下腔 Repeated transplantation of bone marrow stromal cells into subarachnoid space to promote spinal cord injury repair LI Huiying,WEN Yimin,CHEN Keming,et al. The Second Clinic Hospital,Lanzhou University, Gansu 730018，China Abstract：［Objective］To investigate the effect of multiple subarachnoid infusion of bone marrow stromal cells(BMSCs) on spinal cord injury repair in adult Wistar rats.［Method］The BMSCs were cultured, purified and labeled with Hoecst33342 in vitro. After the plete spinal cord injury rat model at T10T11 level was successfully established, sixty injured rats were randomly divided into 4 groups: groups A, B, C, and D. The labeled BMSCs in a dose of 1106 / ml were injected into subarachnoid space of the rats via a catheters under the aubarachnoid space in group A,B and C. The cells were administered only once in group A 1week after injury. However, they were injected after injury 1 and 3 weeks in group B and 1, 3 and 5 weeks after injury in group C, respectively. Neurological functions were evaluated using the BassoBeattieBresnahan (BBB) Scale 1, 3，7，9 and 12 weeks after transplantation. The immunohistochemistry and fluorescence microscopy were analyzed to observe the migration, survival, differentiation and histomorphology of BMSCs. ［Result］The transpl
公司管理相關(guān)推薦
鄂ICP備17016276號-1